| 均匀设计法优选柿叶总黄酮的提取工艺 |
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| 引用本文： | 陈丽,韦敏.均匀设计法优选柿叶总黄酮的提取工艺[J].中国药房,2012(7):606-608. |
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| 作者姓名： | 陈丽 韦敏 |
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| 作者单位： | 柳州医学高等专科学校药学教研室;柳州市人民医院药剂科 |
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| 基金项目： | 广西教育厅科研立项项目(201106LX736) |
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| 摘 要： | 目的:优选柿叶总黄酮的提取工艺。方法:采用碱溶酸沉与超声波相结合的方法提取柿叶总黄酮,并以柿叶总黄酮含量为评价指标,用均匀设计法优选提取工艺。结果:最佳工艺条件为15%HCl调pH值为6,20%NaOH调pH值为10,液料比为11:1,超声时间为32min,超声温度为30℃,乙醇浓度为70%。结论:优选工艺简便、快速、准确,适用于柿叶总黄酮的提取。
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| 关 键 词： | 柿叶 总黄酮 均匀设计 超声波提取 碱溶酸沉法 |
Optimization of the Extraction Technology of Persimmon Leaf Total Flavonids by Uniform Design |
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| CHEN LiPharmacy Teaching and Research Section,Liuzhou Medical College,Guangxi Liuzhou,China WEI Min.Optimization of the Extraction Technology of Persimmon Leaf Total Flavonids by Uniform Design[J].China Pharmacy,2012(7):606-608. |
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| Authors: | CHEN LiPharmacy Teaching and Research Section Liuzhou Medical College Guangxi Liuzhou China WEI Min |
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| Institution: | CHEN Li(Pharmacy Teaching and Research Section,Liuzhou Medical College,Guangxi Liuzhou 545005,China) WEI Min(Dept.of Pharmacy,Liuzhou People’s Hospital,Guangxi Liuzhou 545005,China) |
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| Abstract: | OBJECTIVE：To optimize the extraction technology of total flavonoids from persimmon leaf.METHODS：Alkali-solution and acid-isolation and ultrasonic extraction techhology were used to extract alkali-solution and acid-isolation.The extraction technology was optimized by uniform design using the content of persimmon leaf total flavonids as index.RESULTS：The optimal extraction conditions were as follows：15% HCL？to adjust pH？as？6,20% NaOH？to adjust pH？as 10,the ratio of liquid to material was 11：1,the ultrasonic？time scheduled for？32min,the ultrasonic？temperature？set at？30 ℃,the extraction solvent was 70% ethanol.CONCLUSION：The optimum extraction technology is simple,rapid and accurate.It is suitable for？the extraction？of total flavonoids from persimmon leaf. |
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| Keywords: | Persimmon leaf Total flavonoids Uniform design Ultrasonic extraction Alkali-solution and acid-isolation method |
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