细胞色素C对柔红霉素诱导AML细胞凋亡的影响

为了研究细胞色素C体外作用急性非淋巴细胞性白血病(AML)细胞的效应及其对柔红霉素(DNR)诱导 AML细胞凋亡的影响,分别采用瑞氏-姬姆萨染色和硝基四氰唑蓝还原试验观察细胞色素C引起AML细胞的分化 效应;用流式细胞术检测和荧光染色方法检测AML细胞的凋亡效应。结果表明:①不同浓度的细胞色素C单独作 用AML细胞产生的效应不同:浓度为10μl／ml的细胞色素C作用AML细胞可产生分化效应;浓度为20μl/ml的 细胞色素C作用AML细胞,产生明显的凋亡效应;浓度为40μ/ml的细胞色素C作用AML细胞,可导致细胞的坏 死。②浓度为10.0μg／ml的细胞色素C预先作用AML细胞导致DNR诱导的细胞凋亡率明显降低(P<0.01),浓 度为20.0μg／ml的细胞色素C预先作用对DNR诱导的细胞凋亡率影响不大(P>0.05)。③如果DNR预先作用 AML细胞后再经细胞色素C处理,两种浓度的细胞色素C均可使DNR诱导的细胞凋亡率明显增高(P<0.01)。 结论:细胞色素C对柔红霉素(DNR)诱导AML细胞凋亡可能有一定的影响。

Influence of Cytochrome C on Apoptosis Induced by Daunorubicine in Acute Myeloid Leukemia (AML) Cells

The purpose was to study the responses of AML cell treated with cytochrome C and to explore the influence of cytochrome C on apoptosis of AML cell induced by daunorudicine (DNR). The differentiation of AML cell was detected by Wright-Giemsa staining and NBT test, the apoptosis of AML cell was assayed by flow cytometry and fluorescence microscopy. The results showed as follows: (1) different concentrations of cytochrome C could induce different effects on AML cells. Concentration of cytochrome C for differentiation was 10 microl/ml, for apoptosis was 20 microl/ml, and for necrosis was 40 microl/ml. (2) the apoptosis of AML cells decreased with the administration of cytochrome C in 10.0 microg/ml before treating AML cells with DNR (P < 0.01), but no change was shown with the administration of cytochrome C in 20.0 microg/ml (P > 0.05). (3) in reverse sequence, administrating of cytochrome C in 10 microl/ml and 20 microl/ml after treating AML cells with DNR, two different concentrations of cytochrome C could increase the apoptosis of AML cells (P < 0.01). It is suggested that cytochrome C may probably affect the apoptosis of AML cells induced by DNR.