脆性组胺酸三聚体蛋白在上皮性卵巢肿瘤中的表达

目的 :探讨脆性组胺酸三聚体 (FHIT)蛋白在上皮性卵巢肿瘤组织中的表达及其意义 ,分析FHIT与P5 3蛋白、nm2 3 H1产物表达之间的相关性。方法 :应用免疫组化二步法检测 83例上皮性卵巢肿瘤蜡块组织标本中FHIT蛋白、P5 3蛋白及nm2 3 H1产物的表达。结果 :FHIT蛋白缺失仅见于恶性上皮性卵巢肿瘤 ,缺失率为 17.0 %。在良性与交界性上皮性卵巢肿瘤中未见FHIT蛋白缺失。在上皮性卵巢癌不同组织学分级中FHIT蛋白的缺失率分别为G10 / 6、G2 8.0 %、G331.8% ,低分化肿瘤FHIT蛋白缺失率显著高于高中分化肿瘤 (P <0 .0 5 ) ;在不同组织学类型中 ,FHIT蛋白缺失仅见于浆液性癌和透明细胞癌 ,缺失率分别为 2 5 .0 %和 1/ 3,在粘液性癌和子宫内膜样癌中未见FHIT蛋白缺失 ,但差异无显著性 (P >0 .0 5 ) ;FHIT蛋白缺失与淋巴结转移、残留癌灶大小及nm2 3 H1产物表达有明显相关性 ,与FIGO分期、有无腹水及P5 3蛋白表达无明显相关性。结论 :FHIT蛋白缺失是恶性上皮性卵巢肿瘤的特征 ,FHIT蛋白表达缺失在卵巢肿瘤的发生发展过程中有一定的作用 ,可能与上皮性卵巢癌的恶性进展及转移有关

The expression of FHIT gene in epithelial ovarian tumors

Objective:To investigate the expression of fragile histidine triad (FHIT) protein,its clinical implication in epithelial ovarian tumor and the possible relationship with the expression of P53 and nm23 H1.Method:The expressions of FHIT,P53 and nm23 H1 proteins were determined by immunohistochemistry in 53 epithelial ovarian carcinomas,16 borderline tumors and 14 benign tumors.Results:9 cases of epithelial ovarian cancer (17%) showed marked loss or absence of FHIT expression but there were no loss of FHIT protein in benign and borderline tumors.Notably,impaired expression of FHIT protein occurred only in carcinomas of grade 2 and 3 (8.0% and 31.8%,respectively) and there was no loss of FHIT protein in carcinomas of grade 1.Lower expression of FHIT was observed in serous adenocarcinoma and clear cell carcinoma but not in mucinous adenocarcinoma and endometrioid carcinoma.FHIT expression was significantly related with lymph node metastasis,the size of residual tumor after operation and the expression of nm23 H1 gene.However,there was no significant correlation between the expression of FHIT gene and the FIGO stage,the existence of ascites or the expression of P53 protein.Conclusion:Loss of FHIT protein characterizes epithelial ovarian cancer.Decreased FHIT expression may play an important role in the progression and metastasis of the epithelial ovarian cancers.