正交试验法优选加味四君子汤提取工艺

目的： 优选加味四君子汤的提取工艺。 方法： 以人参皂苷总提取量为指标，采用L₉（3⁴）正交试验考察乙醇体积分数及用量、提取次数、提取时间对醇提工艺的影响；以总多糖得率为指标，通过正交试验考察料液比、提取次数、提取时间对水提工艺的影响。采用HPLC测定人参皂苷含量，流动相乙腈-水（13：87），检测波长203 nm；利用UV测定总多糖含量，检测波长489 nm。 结果： 最佳醇提工艺为加6倍量70%乙醇提取2次，每次1.5 h；人参皂苷提取量达7.69 mg·g^-1。最佳水提工艺为加8倍量水提取3次，每次2 h；总多糖得率达2.18%。 结论： 优选的提取工艺稳定可行，可充分提取加味四君子汤中醇溶性和水溶性有效成分。

Optimization of Extraction Technology for Modified Sijunzi Decoction by Orthogonal Design

Objective: To optimize extraction technology of modified Sijunzi decoction. Method: Taking total extraction amounts of ginsenoside as index,L₉(3⁴) orthogonal design was adopted to optimize alcohol extraction technology of Ginseng Radix et Rhizoma,Atractylodis Macrocephalae Rhizoma and Paeoniae Radix Alba with ethanol amount and concentration,extraction time and times as factors;With yield of total polysaccharides as index,L₉(3⁴) orthogonal design was used to optimize water extraction technology of Poriae Cutis,Hedyotidis Herba and Glycyrrhizae Radix et Rhizoma by taking solid-liquid ratio,extraction time and times as factors.The content of ginsenoside was determined by HPLC,mobile phase of acetonitrile-water(13:87),detection wavelength 203 nm;UV was adopted to measure the content of polysaccharides with detection wavelength at 489 nm. Result: Optimum alcohol extraction process was as following:extracted twice with 6 times the amount of 70% ethanol for 1.5 h per time;Extraction amount of ginsenoside was up to 7.69 mg·g^-1.Optimum water extraction process was:extracted three times with 8 times the amount of water for 2 h each time;Yield of total polysaccharides reached 2.18%. Conclusion: This optimized extraction technology was stable and feasible,it could full extract alcohol-soluble and water-soluble active ingredients from modified Sijunzi decoction.