丁酸钠对醋酸诱导的大鼠结肠炎的治疗作用

目的 :研究丁酸钠灌肠治疗对结肠炎临床表现、粘膜损伤的改善作用及其与凋亡有关的可能机制。方法 :用醋酸诱导大鼠结肠炎 ,3d后随机分为三组 ,每天分别给予丁酸钠、5 氨基水杨酸和 0 .9%氯化钠溶液灌肠 1次。于第11天处死 ,进行结肠损伤指数评分及组织还原型谷胱甘肽 (GSH)和黄嘌呤氧化酶 (XOD)的测定。结果 :0 .9%氯化钠溶液灌肠组 ,腹泻及结肠损伤持续存在 ,结肠损伤指数明显高于丁酸钠灌肠治疗组和 5 氨基水杨酸灌肠治疗组。而丁酸钠灌肠治疗组 ,腹泻及结肠均恢复正常 ,且均与组织的GSH、XOD水平相关。结论 :丁酸盐灌肠治疗可刺激结肠损伤修复 ,表现为临床症状好转 ,炎症抑制及相应指标改善。提示丁酸盐治疗溃疡性结肠炎的广阔前景。

Effect of Butyrate Enema on Experimental Rat Colitis Induced with Acetic Acid

Objective:To examine the impact of butyrate enema on the clinical manifestation, severity of inflammation, and the probable mechanism related to apoptosis of colonic cells. Method:Distal colitis was induced with an acetic acid enema in rats. Three days later, rats were randomly divided into three groups to receive:40 mmol/L sodium butyrate enema, 5 acetylsalicyclic acid (5 ASA) enema, or normal saline enema daily, respectively. All rats were put to death on day 11, colonic damage score and tissue redued glutathione (GSH) as well as xanthine oxidase (XOD) activities were measured. Colonic tissues were also examined by immunohistochemistry. Results:In normal saline enema group, diarrhea and extensive colonic damage existed persistently. The colonic damage score in normal saline enema group was obviously higher than those in sodium butyrate enema group or in 5 ASA enema group. In contrast, in the sodium butyrate enema group, diarrhea ceased, colonic damage score had improved, and tissue reduced GSH and XOD activities had recovered. Conclusion:Sodium butyrate enema resulting in colonic repair had confirmed from clinical recovery.