| 睡眠剥夺应激对大鼠海马的损伤作用及其对探究行为的影响 |
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| 引用本文： | 王新兴,梅竹松,刘伟丽,张志清,武磊,弓景波,钱令嘉.睡眠剥夺应激对大鼠海马的损伤作用及其对探究行为的影响[J].军事医学科学院院刊,2011,35(9):663-665,680. |
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| 作者姓名： | 王新兴 梅竹松 刘伟丽 张志清 武磊 弓景波 钱令嘉 |
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| 作者单位： | 1. 军事医学科学院基础医学研究所全军军事认知与心理卫生研究中心,北京,100850
2. 军事医学科学院卫生学环境医学研究所,天津,300050 |
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| 基金项目： | 国家科技重大专项(2008ZXJ090-011); 国家自然科学基金青年项目(81000584) |
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| 摘 要： | 目的观察睡眠剥夺应激对大鼠海马的损伤作用,并研究其对大鼠探究行为的影响。方法以改良多平台水环境（MMPM）建立睡眠剥夺模型,设大平台对照组和睡眠剥夺组。分别记录各组大鼠体质量、血浆糖皮质激素（GC）和神经元特异性烯醇化酶（NSE）水平以及乳酸脱氢酶（LDH）活性的变化;常规HE染色法观察海马损伤情况;旷场实验确定大鼠自主探究能力。结果睡眠剥夺5 d和7 d后大鼠血浆GC含量和LDH活性均显著高于对照组。睡眠剥夺3 d后血浆中NSE水平明显高于对照组,并有随时间延长而逐渐升高的趋势。病理染色和旷场实验显示,睡眠剥夺3 d后大鼠即出现海马损伤和探究行为评分降低。结论睡眠剥夺对大鼠海马具有明显的损伤作用,可能是睡眠剥夺大鼠自主探究行为下降的重要原因。
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| 关 键 词： | 睡眠剥夺 海马损伤 探究行为 |
Adverse effect of sleep deprivation on hippocampus and exploratory behavior in rats |
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| WANG Xin-xing,MEI Zhu-song,LIU Wei-li,ZHANG Zhi-qing,WU Lei,GONG Jing-bo,QIAN Ling-jia.Adverse effect of sleep deprivation on hippocampus and exploratory behavior in rats[J].Bulletin of the Academy of Military Medical Sciences,2011,35(9):663-665,680. |
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| Authors: | WANG Xin-xing MEI Zhu-song LIU Wei-li ZHANG Zhi-qing WU Lei GONG Jing-bo QIAN Ling-jia |
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| Institution: | WANG Xin-xing1,MEI Zhu-song1,LIU Wei-li1,ZHANG Zhi-qing2,WU Lei2,GONG Jing-bo1,QIAN Ling-jia1*(1.Institute of Basic Medical Sciences,Research Center for Military Cognition and Mental Hygiene of PLA,Beijing 100850,China,2.Institute of Health and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,China) |
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| Abstract: | Objective To observe the effect of sleep deprivation on hippocampus and exploratory behavior in rats.Methods The modified multiple platform method(MMPM) was used to establish sleep deprivation model.Open-field test was applied to evaluate the behavior of the model rats.Results The sleep deprivation rats showed decreased body massin the 3rd,5th and 7th day compared with the control groups.The glucocorticoid(GC) content and lactate dehydrogenase(LDH) activity in the plasma of the sleep deprivation rats showed... |
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| Keywords: | sleep deprivation hippocampus injury exploratory behavior |
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