子宫内膜异位症患者子宫内膜HIF-1α、CD34、CD54的表达及相互关系

目的观察缺氧诱导因子1α(HIF-1α)、CD34、CD54与子宫内膜异位症(EMS)发生的关系,以期探讨EMS的发病机制。方法选取26例EMS手术患者,所有病例均经腹腔镜或开腹手术确诊,经病理证实为腹膜型EMS 8例,卵巢子宫内膜异位囊肿9例,子宫腺肌症9例,同期选取子宫肌瘤手术患者6例为对照组,应用免疫组织化学法(SP)测定2组子宫内膜组织中HIF-1α、CD34、CD54的表达。结果 HIF-1α、CD34、CD54在腹膜型EMS组、卵巢子宫内膜异位囊肿组、子宫腺肌症组的表达均高于对照组,差异有统计学意义(P0.05)。HIF-1α与CD34、CD54的表达呈正相关。结论 HIF-1α可能在EMS异位病灶形成中起重要作用,推测内异症的发生与局部病灶缺氧有关。且HIF-1α与CD34的表达呈正相关,提示HIF-1α可以促进内异症组织血管生成;HIF-1α与CD54的表达呈正相关,推测HIF-1α可加强异位内膜细胞的黏附作用,从而有利于形成异位灶。

The expression and correlation of HIF-1α, CD34 and CD54 in endometrium of patients with endometriosis

Objective To observe the relationship between hypoxia-inducible factor 1 alpha (HIF-1α), CD34, CD54 and endometriosis (EMS), in order to explore the pathogenesis of EMS.Methods 26 EMS surgery patients were selected, all cases were diagnosed by laparoscopy or laparotomy.Confirmed by pathology, 8 cases were peritoneal EMS, 9 cases were ovarian endometriosis cyst and 9 cases were adenomyosis.Six patients with uterine fibroids surgery were selected as control group at the same time.The expression of HIF-1α, CD34, CD54 in endometrial tissues were detected by streptavidin-perosidase (SP) in two groups.Results The expression of HIF-1α, CD34, CD54 in peritoneal EMS group, ovarian endometriosis cyst group and adenomyosis group was higher than that in control group, with statistical difference (P<0.05).HIF-1α was positively correlated with the expression of CD34 and CD54.Conclusion HIF-1α may play an important role in the formation of EMS ectopic lesions,speculating that the occurrence of endometriosis related to hypoxia in local lesions.Moreover, HIF-1α was positively correlated with the expression of CD34 suggesting that HIF-1α can promote angiogenesis in EMS tissue.HIF-1α was positively correlated with the expression of CD54 suggesting that HIF-1α can enhance the adhesion of ectopic endometrial cells, thus which is conducive to the formation of ectopic lesions.