三七总皂苷联用阿司匹林对后者抗血小板凝集作用的影响

目的:对比与三七总皂苷联用前后阿司匹林的抗血小板凝集作用,探讨三七总皂苷对阿司匹林药效的影响。方法:将24只大鼠随机分为4组,分别为正常组,三七总皂苷组(NTS,31.25 mg·kg~(-1)),阿司匹林组(ASA,20.83 mg·kg~(-1)),三七总皂苷与阿司匹林联用组(NTS,31.25 mg·kg~(-1)+ASA,20.83 mg·kg~(-1))。除正常组外,其余各组分别灌胃给药相应浓度药液。于第5,30,60,120,240,480 min时取血,采用酶联免疫吸附测定(ELISA)测定血清中血栓素A_2(TXA_2),环氧化酶-1(COX-1),环氧化酶-2(COX-2)水平。结果:ASA组COX-1及COX-2含量与正常组无显著性差异。NTS组在5~240 min内COX-1含量显著低于正常组(P0.01),在30,60,120,480 min COX-2含量显著低于正常组(P0.01)。ASA与NTS联用组在5~60 min内COX-1含量显著低于正常组及ASA组(P0.05),在5~240 min内COX-2含量显著低于正常组和ASA组(P0.01)。各实验组大鼠血清中TXA_2含量在5~240 min内均显著低于正常组(P0.01),其中联用组在第30,60,240 min明显低于阿司匹林组(P0.05,P0.01)。联用组对TXA_2的抑制作用在60 min时达到峰值。结论:三七总皂苷与阿司匹林联用后抗血小板凝集作用优于阿司匹林或三七总皂苷单用组。二者对TXA_2的抑制为相加作用,其中三七总皂苷可抑制COX-1,COX-2的表达,可能是增强阿司匹林抗血小板凝集作用的途径之一。

Effect of Notoginseng Total Saponins Combined with Aspirin on Platelet Aggregation

Objective: To compare the effect of aspirin on antiplatelet aggregation before and after the combined administration with Notoginseng total saponins, in order to investigate the effect of Notoginseng total saponins on the efficacy of aspirin. Method: A total of 24 rats were randomly divided into 4 groups:control group, Notoginseng total saponins group (NTS, 31.25 mg · kg^-1), aspirin group (ASA, 20.83 mg · kg^-1), and combination group (NTS, 31.25 mg · kg^-1 +ASA, 20.83 mg · kg^-1). All of the experimental groups were intragastrically administered with the corresponding concentrations of liquid, except for control group. Blood samples were taken at 5, 30, 60, 120, 240, 480 min, the levels of thromboxane A₂ (TXA₂), cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) in serum were determined by enzyme-linked immunosurbent assay(ELISA). Result: The contents of COX-1 and COX-2 in ASA group and blank control group showed no significant difference. In group NTS, the content of COX-1 between 5-240 min was significantly lower than that in the blank group (P<0.01), and the content of COX-2 was significantly lower than that in the blank group (P<0.01) at 30, 60,120,480 min after drug administration. The COX-1 content of ASA and NTS combination group between 5-60 min was significantly lower than that in blank group and ASA group (P<0.05), and the COX-2 content between 5-240 min was significantly lower than that in blank group and ASA group (P<0.01). The content of TXA₂ in serum of each experimental group was significantly lower than that in blank control group (P<0.05) between 5-240 min, in which that of combination group were significantly lower than that in aspirin group (P<0.05) at 30 min, 60 min and 240 min after drug administration. The inhibitory effect of the combination group on TXA₂ reached a peak at 60 min after administration. Conclusion: The combined administration of NTS and aspirin showed a better effect in antiplatelet aggregation than the single administration of aspirin or NTS. Their inhibitory effect on TXA₂ is an additive action, in which NTS can inhibit the expressions of COX-1 and COX-2, which may be one of the ways to enhance aspirin''s anti-platelet aggregation.