In vitro synthesis of human IgE is suppressed by human IgG |
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Authors: | H. Haas M. Schlaak |
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Affiliation: | (1) Forschungsinstitut Borstel, Borstel, Germany |
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Abstract: | Recently it has been shown that intravenous immunoglobulin (IVIG) preparations suppress thein vitro synthesis of IgG, IgA, and IgM. In this paper we demonstrate that IVIG and IgG purified from a single donor's serum also suppress thein vitro synthesis of IgE. We had noticed this effect when we added human serum (HS) toin vitro cultures for IgE synthesis. The interleukin-4 (IL-4)-induced IgE synthesis from human peripheral blood mononuclear cells (PBMC) in the presence of fetal calf serum (FCS) was suppressed by HS in a dose-dependent fashion. The following results indicate that this suppression is mediated by IgG: (1) IVIG preparations, which consist mainly of IgG, suppressed the IgE synthesis from IL-4-stimulated PBMC in a dose-dependent way; (2) when HS was fractionated by protein G sepharose or anti-IgG sepharose, the eluate fractions (containing IgG), but not the effluent fractions (void of IgG) suppressed IgE synthesis, whereas the opposite was found when HS was fractionated by FCS-coupled sepharose. We conclude from these data that human IgG preparations suppress thein vitro synthesis not only of the IgG, IgA, and IgM isotypes, but also of the IgE isotype. |
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Keywords: | IgE IgG intravenous immunoglobulin interleukin-4 |
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