| 紫外-可见分光光度法测定振源口服液(无糖型)中人参总皂苷的含量 |
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| 引用本文： | 庞威,赵宏峰,弥宏,越皓,曲芯瑶,郭禹彤.紫外-可见分光光度法测定振源口服液(无糖型)中人参总皂苷的含量[J].中国医药指南,2014(29):59-60. |
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| 作者姓名： | 庞威 赵宏峰 弥宏 越皓 曲芯瑶 郭禹彤 |
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| 作者单位： | 长春中医药大学;吉林省中医药科学院 |
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| 基金项目： | 吉林省科技厅技术成果转化重大项目（20115090） |
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| 摘 要： | 目的采用紫外-可见分光光度法测定振源口服液(无糖型)中人参总皂苷的含量。方法以人参皂苷Re为对照,用1%香草醛-高氯酸试液及77%硫酸显色,在540 nm波长下通过紫外分光光度法对人参总皂苷进行含量测定。结果人参皂苷Re浓度在6.6566.55μg/m L范围内线性关系良好,回归方程A=-0.0751+0.0195X(相关系数r=0.9995),平均回收率为96.92%(RSD=1.79%)。结论该方法简便可靠,稳定性好,可用于本品的含量测定和质量控制。
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| 关 键 词： | 紫外-可见分光光度法 振源口服液(无糖型) 人参皂苷Re 人参总皂苷 含量测定 |
Determination of Total Ginsenosides in Free-sugar Zhenyuan Oral Liquid by Ultraviolet-visible Spectrophotometry |
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| Institution: | PANG Wei, ZHAO Hong-feng, MI Hong, YUE Hao, QU Xin-yao, GUO Yu-tong (Changchun University of Chinese Medicine, Changehun 130117, China, Jilin Academy of Traditional Chinese Medicine, Changchun 130012, China) |
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| Abstract: | Objevtive To determine the content of total Ginsenosides in free-sugar Zhenyuan oral liquid by ultraviolet-visible spectrophotometry. Method Ginsenoside Re was selected as control article, coloration was used with 1%vanillin-perchloric acid and 77%sulfuric acid, the content of total Ginsenosides in free-sugar Zhenyuan oral liquid was determined at 540 nm by UV. Result The calibration curve was linear over the range of 6.65-66.55μg/mL with the correlation of 0.9995, and the linear regression equation was A=-0.0751＋0.0195X, the average recovery of Ginsenoside Re was 96.92%with RSD of 1.79%（n=6）. Conclusion The method was simple, reliable and stable, so it is suitable for content determination and quality control of total Ginsenosides in free-sugar Zhenyuan oral liquid. |
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| Keywords: | Ultraviolet-visible spectrophotometry Free-sugar Zhenyuan oral liquid Ginsenoside Re Total Ginsenosides Content assay |
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