pCDNA3.0载体介导METH1基因对人脐静脉内皮细胞生长的抑制

目的研究METH1基因对人脐静脉内皮细胞(HUVEC)体外生长的抑制作用。方法构建真核表达载体pCDNA3.0-METH1,转染肝癌细胞系HepG2中。体外扩增HUVEC,采用MTT法检测HepG2/METH1及HepG2培养的上清对HUVEC增殖的影响。结果成功的构建了真核表达载体pCDNA3.0-METH1,并在HepG2中稳定表达。MTT法结果显示与对照组相比,HepG2/METH1组对HUVEC增殖有明显的抑制作用,并呈剂量依赖性(P<0.01);而HepG2组对HUVEC增殖有明显的促进作用,呈剂量依赖性(P<0.05)。结论pCDNA3.0-METH1对HUVEC体外生长具有明显的抑制作用,提示METH1对瘢痕内血管的抑制治疗具有潜在的临床应用价值。

Inhibitory effects of pCDNA3.0 vector expression of METH1 gene on human umbilical vein endothelial cells

Objective To explore the growth inhibitory effects of pCDNA3.0 vector expression of METH1 gene on human umbilical vein endothelial cells(HUVEC) in vitro. Methods To construct eukaryotic vector pCDNA3.0 expressing METH1 and transfect the vector to HepG2 cells by LipofectamineTM reagent. HUVEC were expanded in vitro, MTT method was used to examine the effects of HepG2/ METH1 and HepG2 supernatant on HUVEC proliferation. Results Eukaryotic vector pCDNA3.0 expressing METH1 was successfully constructed, and HepG2 cells expressing METH1 stably were got after transfection.The result of MTT showed that the group of HepG2/METH1 inhibited the proliferation of HUVEC with dose-depended.Whereas,the group of HepG2 accelerated the proliferation of HUVEC with dose-depended. Conclusion pCDNA3.0 vector expressing METH1 gene can inhibit the growth of HUVEC in vitro. This result indicate that METH1 has potential prospect of clinical application on inhibition of vessel growth in scar.