H_2O_2诱导肝细胞凋亡时p53、bcl-2mRNA的表达及其意义

目的　探讨H2 O2 诱导人类正常肝细胞系 (L0 2细胞 )凋亡时p5 3、bcl 2mRNA表达的意义及丹参有效成分Rxa对其表达的影响。方法　以正常L0 2细胞为对照 (L组 ) ,以 10 μmol/LH2 O2 诱导L0 2细胞凋亡为细胞模型 (LH组 ) ,观察Rxa处理 (LaH组 ,Rxa 2mmol/L)对 p5 3、bcl 2mRNA表达 (原位杂交结合图像分析处理 )的影响。结果　LH组 2～ 6hp5 3mRNA表达随凋亡细胞指数增高而增强 ,6～ 8h表达下降 ;bcl 2mRNA表达弱。相比较LaH组各时限检测点p5 3mRNA表达均低于LH组 ,bcl 2mRNA表达较强 (P <0 .0 1)。结论　Rxa可能通过 p5 3的细胞周期调控、DNA损伤修复作用和bcl 2的抗凋亡作用减轻L0 2细胞过氧化损伤。

The expression of p53 and bcl-2 mRNA in H_2O_2-induced apoptotic human cultured hepatocytes (L02 cells) and its implication

Objective To evaluate the significance of changes in expression of p53 and bcl-2 mRNA in H_2O_2-induced apoptotic human cultured hepatocytes (L02 cells) and the effect of Rxa (extract from Radix Salviae Miltiorrhizae) on it. Methods L02 cells served as control group (L group). H_2O_2 of 10 umol/L was used to induce L02 cell a poptosis to construct the cell model (LH group). The effects of Rxa (LaH group, 2mmol/L Rxa) on the expression of p53 and bcl-2 mRNA were observed by using i n situ hybrization in combined with imaging analysis process. Results In the LH group, the expression of p53 mRNA wa s raised in accordance with the increase of apoptostic index (AI) within 2h to 6h and then decreased gradually, and the expression of bcl-2 mRNA was very w eak. But in the LaH group, the expression of p53 and bcl-2 mRNA at different ti me points was lower than in the LH group, and bcl-2 mRNA showed a strong expres sion (P<0.01).Conclusion Rxa could reduce the H_2O_2-induced injuri es o f L02 cells via regulating cell-cycle, repairing DNA injuries by p53 in conjun ction with the apoptotic property of bcl-2.