基于“回阳救逆”功效附子对小鼠心电图影响的“效—毒”关联评价

目的:基于"回阳救逆"功效研究附子对小鼠心电图的影响,探讨附子药效和毒性的关联性。方法:灌胃给予小鼠不同剂量的附子各组分后,观察小鼠的心电图变化,检测血清中AST、LDH、cTn-I的含量以及心体比值变化。结果:附子各组分引起心电图QRS间期延长和S-T段抬高,血清AST、LDH、cTn-I的含量依剂量呈现不同程度的升高,以醇提组分的升高幅度显著,从药效剂量的9 g开始呈现出一定毒副作用。结论:附子药效剂量会引起小鼠心电图室早的改变和心肌损伤,缺血的血清心肌酶含量升高,必须重视附子药效剂量下毒副作用的监测工作,研究在"回阳救逆"功效下的药效作用发挥和毒性作用表达的关联性,使药效作用发挥达到最优话,毒副作用产生降到最低值,使附子的临床应用更具安全性和有效性。

Efficacy-Toxicity Relevancy of Fuzi regarding its Effect of Restoring Yang and Saving from Collapse

Objective：To evaluate the impact of Fuzi on ECG result of mice to explore the relevancy between efficacy and toxicity of Fuzi regarding its effect of restoring yang and saving from collapse .Methods：Different doses of Fuzi were given to mice by gavage , then ob-served the changes of ECG , measured AST, LDH, cTn-I and the change of heart body ratio .Results：Various Fuzi extract can lengthen interphase of QRS, elevate the segment of S-T, and increase AST, LDH and cTn-I according to the doses.alcohol extracts raised most significantly, and presented side effects at dose of 9 g.Conclusion：Fuzi’ efficacy dose will cause ECG changes and myocardial injury , so monitoring toxicity below the efficacy doses should be paid attention to .To research the relevancy between efficacy and toxicity regard-ing the“Restoring yang and saving from collapse” effect can minimize toxicity and give full play to efficacy , so that Fuzi has better safety and efficacy .