通腑泻肺方对ALI/ARDS大鼠肺肠功能的影响

目的:观察通腑泻肺方对ALI/ARDS大鼠肺、肠功能的影响,为"肺与大肠相表里"理论提供实验证据。方法:健康SD大鼠30只,随机分为3组,正常组、模型组和治疗组,采用尾静脉注射油酸加内毒素方法造成ALI/ARDS模型,治疗组给予通腑泻肺中药,于实验第7天检测分析各组大鼠肺功能、肠屏障功能指标。结果:1)与正常组比较,模型组大鼠肺顺应性显著降低、吸气和呼气阻力显著升高(P0.01),治疗后肺顺应性、吸气阻力有所改善;2)模型组大鼠肠组织中ocdudin蛋白表达较正常组明显降低(P0.01),治疗后显著升高(P0.05);SIgA含量较正常组明显升高(P0.01),治疗后显著降低(P0.05)。结论:除肺通气功能障碍外,ALI/ARDS大鼠肠屏障功能同时受损。通腑泻肺中药能改善肺通气障碍,保护肠黏膜屏障功能。

Effects of Tongfu Xiefei Herbs on Lung and Large Intestinal Function in ALI/ARDS Rats

Objective：To observe the effects of Tongfu Xiefei Herbs on lung and large intestinal function in ALI /ARDS rats, then pro-vide experimental basis for ALI/ARDS lung and large intestine concurrent therapy .Methods：Thirty healthy SD rats were randomly di-vided into 3 groups：control group , model group , treatment group .The ARDS rats was established by intravenous injecting oleic acid plus endotoxin in caudal vein , and treatment group were treated with Tongfu Xiefei herbs .The indicators of lung and intestinal function in each group were observed after 7 days.Results：1）Compared with control group, the lung compliance of model group was obviously decreased;the aspiratory and expiratory resistance were obviously increased （P＆lt;0.01）.The lung function of treatment group improved . 2）Occludin protein expression in large intestine of model group were obviously decreased than that of control group （P＆lt;0.01）,and were significantly increased after treatment （P＆lt;0.05）.The SIgA of model group were obviously increased than control group （P＆lt;0.01）,and were significantly decreased after treatment （P＆lt;0.05）.Conclusion：With ventilation dysfunction, ALI/ARDS intestinal barrier function was damaged at the same time .Tongfu Xiefei herbs could improve the lung function and protect intestinal barrier function .