HPLC法同时测定中药菟丝子中五种黄酮类成分含量的研究

目的:建立中药菟丝子中金丝桃苷、紫云英苷、槲皮素、山柰酚和异鼠李素的含量的高效液相色谱测定方法。方法:采用Agilent TC C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.4%磷酸,紫外检测器:检测波长360 nm,流速为1 mL/min,梯度洗脱:0→60 min→80 min,甲醇的比例由25%→40%→60%,柱温45℃,测定中药菟丝子中5种黄酮类成分的含量。结果:色谱峰分离情况良好,菟丝子中5种化学成分在各自的浓度范围内均具有良好的线性关系;加样回收率为98.0%~102.2%,RSD为1.44%~2.63%。结论:所建方法简便、准确,重现性好,可用于菟丝子药材的质量评价。

Study on Simultaneous Determination of Five Kinds of Flavonoids in Seed of Semen Cuscutae by HPLC

Objective： To determine the content of five kinds of flavonoids in the seed of semen cuscutae, including hyperoside, astraga- lin, quercetin, kaempferol and isorhamnetin. Methods： The HPLC method was used to determine the content of flavonoids in the seeds of semen cuseutae. The analysis was carried out on Agilent TC C18 column （250 mm ×4. 6 mm, 5 μm）, with MeOH-0. 4 % H3PO4as mobile phase, the flow rate of 1 mL · min-l and wave length at 360 nm. The gradient elute time was 0→min→80min, and the proportion of methanol was 25%→40%→60%. at45℃. Results： Chromatographic peak separation was in good condition, and five kinds of flavonoids had good linear relationship respectively within their own scope of concentration ; the recovery was 98.0% ~ 98. 0%, RSD was 1.44% ~ 2.63%. Conclusion：The established method is simple, accurate, and reproducible and can be used as the quality evaluation of semen cuscutae.