消岩汤剂拆方配伍对A549肺腺癌细胞体外生长抑制作用研究

目的] 探讨消岩汤及各拆方组对人肺腺癌 A549细胞的体外生长的作用机制,探究消岩汤剂中最有效的作用成分和浓度。方法] 消岩汤全方和根据功效拆分的清热解毒组、活血化瘀组、益气扶正组,以CCK-8检测A549肺腺癌细胞的细胞增殖活性。结果] 各组均有明显的抑制细胞活性作用,抑制强度依次为:全方组、清热解毒组、活血化瘀组、益气扶正组。结论] 消岩汤及其拆方组在体外实验中均能抑制A549肺腺癌细胞细胞生长,消岩汤全方组当浓度达到50 g/L时达到最大抑制率为85.60%。清热解毒组药物在抗肿瘤作用方面,与全方接近,提示清热解毒组中药为体外实验中全方抗肿瘤作用最有效组分。

Study of the inhibition effect and mechanism of Xiaoyan decoction and decomposed recipe on A549 cells in vitro

Objective] To investigate the inhibition of Xiaoyan decoction and decomposed recipe on A549 in vitro and its possible mechanism. Explore the most effective composition and concentration of Xiaoyan decoction. Methods] According to the effect, Xiaoyan decoction was divided into three groups: Qingre Jiedu group, Huoxue Huayu group and Yiqi Fuzheng group. Use CCK 8 method to detect proliferation activity of A549 lung adenocarcinoma cancer cells. Results] Groups had obvious inhibitory effect of cancer cell activity. The inhibition of strength in the order was Xiaoyan decoction group, Qingre Jiedu group, Huoxue Huayu group and Yiqi Fuzheng group. Conclusion] Xiaoyan decoction and decomposed recipe groups have obvious inhibitory effect on A549 cells activity. Xiaoyan decoction (50 g/L) shows more inhibition on A549 cells with the lowest cell viability and is time-dependent. Effect of Qingre Jiedu group is close to Xiaoyan decoction, and suggests that Qingre Jiedu group is the most effective components for anti-tumor effect of the experiment in vitro of Xiaoyan decoction.