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| VEGF硫代反义寡核苷酸抑制U937细胞VEGF的表达 | |
| 引用本文： | Xiaoyan He Yunjie Tong Min Zhang Ping Zou. VEGF硫代反义寡核苷酸抑制U937细胞VEGF的表达[J]. 中德临床肿瘤学杂志, 2006, 5(6): 420-422. DOI: 10.1007/s10330-005-0425-2 |
| 作者姓名： | Xiaoyan He Yunjie Tong Min Zhang Ping Zou |
| 作者单位： | Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
| 摘 要： | Objective： To study the effect of VEGF antisense oligodeoxynucleotide （VEGF ASODN） on VEGF expression in acute monocyte leukemic cell line U937 in vitro. Methods： U937 cells were incubated with VEGF ASODN （final concentration as follows： 10, 20 and 30 μmol/L respectively） or scrambled sequence, compared with negative control. The expression of VEGF mRNA was measured by semi-quantitative RT-PCR, VEGF protein was measured by Western blot. Results： VEGF ASODN obviously inhibited expression of VEGF mRNA in U937 cell, compared with scrambled sequence and negative control （P〈0.05）. And the inhibition effect was most remarkable after 24 h, which is related with the dose of VEGF ASODN （P〈0.05）. Scrambled sequence groups had no significant difference compared with negative control groups （P〉0.05）. VEGF ASODN obviously inhibited expression of VEGF protein, compared with scrambled sequence and negative control （P〈0.05）. Conclusion： The expressions of VEGF at mRNA and protein levels in leukemic cell line U937 are down-regulated after being treated with VEGF ASODN. |
| 关 键 词： | 抑制效应 血管 生长因子 VEGF表达 U937细胞 |
| 收稿时间： | 2005-08-08 |
| 修稿时间： | 2006-06-25 |
Inhibiting effect of vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotides on VEGF expression in U937 cells |
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| Xiaoyan He,Yunjie Tong,Min Zhang,Ping Zou. Inhibiting effect of vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotides on VEGF expression in U937 cells[J]. The Chinese-German Journal of Clinical Oncology, 2006, 5(6): 420-422. DOI: 10.1007/s10330-005-0425-2 | |
| Authors: | Xiaoyan He Yunjie Tong Min Zhang Ping Zou |
| Affiliation: | (1) Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China |
| Abstract: | Objective To study the effect of VEGF antisense oligodeoxynucleotide (VEGF ASODN) on VEGF expression in acute monocyte leukemic cell line U937 in vitro. Methods U937 cells were incubated with VEGF ASODN (final concentration as follows: 10, 20 and 30 μmol/L respectively) or scrambled sequence, compared with negative control. The expression of VEGF mRNA was measured by semi-quantitative RT-PCR, VEGF protein was measured by Western blot. Results VEGF ASODN obviously inhibited expression of VEGF mRNA in U937 cell, compared with scrambled sequence and negative control (P<0.05). And the inhibition effect was most remarkable after 24 h, which is related with the dose of VEGF ASODN (P<0.05). Scrambled sequence groups had no significant difference compared with negative control groups (P>0.05). VEGF ASODN obviously inhibited expression of VEGF protein, compared with scrambled sequence and negative control (P<0.05). Conclusion The expressions of VEGF at mRNA and protein levels in leukemic cell line U937 are down-regulated after being treated with VEGF ASODN. |
| Keywords: | U937 cell VEGF antisense oligodeoxynucleotide |
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