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| p38分裂原激活蛋白激酶信号转导途径与胃癌细胞长春新碱耐药相关 | |
| 引用本文： | 王雨田,张忠兵,药立波,王吉村,陈苏民,胡家露,樊代明. p38分裂原激活蛋白激酶信号转导途径与胃癌细胞长春新碱耐药相关[J]. 中华消化杂志, 2001, 21(2): 83-85 |
| 作者姓名： | 王雨田 张忠兵 药立波 王吉村 陈苏民 胡家露 樊代明 |
| 作者单位： | 1. 第二军医大学长征医院消化科, 2. 第四军医大学生化教研室 3. 第四军医大学西京医院消化疾病研究所 |
| 基金项目： | 国家自然科学基金(39470785)及全军医药卫生青年基金(96Q078) |
| 摘 要： | 目的 研究p38分型原激活蛋白激酶（MAPK）信号转导途径在胃癌耐药形成中的意义。方法 用免疫共沉淀法及Western bolt法分别研究长春新碱处理胃癌细胞SGC7901及胃癌多药耐药SGC7901／VCR细胞内p38MAPK活性及量的变化。结果 胃癌多药耐药SGC7901／VCR细胞及亲本SGC7901细胞内均存在活性p38MAPK，长春新碱处理此两种细胞均可引起p38MAPK活性增强，后者反应迅速，可在2min内刺激p38MAPK活性增高，而前者反应较慢，至30min时才观察到p38MAPK活性增高。结论38MAPK信号转导途径可能与和长春新碱的抗肿瘤活性相关，肿瘤细胞内p38MAPK对长春新碱刺激的反应速度可能与胃癌长春新碱耐药相关。 |
| 关 键 词： | p38分型原激活蛋白激酶 胃癌 多药耐药 长春新碱 治疗 |
| 修稿时间： | 1999-07-14 |
The effects of vincristine on the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR |
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| WANG Yutian,HU Jialu,YAO Libo,et al.. The effects of vincristine on the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR[J]. Chinese Journal of Digestion, 2001, 21(2): 83-85 | |
| Authors: | WANG Yutian HU Jialu YAO Libo et al. |
| Affiliation: | WANG Yutian,HU Jialu,YAO Libo,et al. Department of gastroenterology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China |
| Abstract: | Objective To study the relation between p38 MAPK pathway and multidrug resistance of stomach cancer. Methods The activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR was examined by immunoprecipitation after treating with vincristine for 0,2,15,30,60 minutes. Results Vincristine could activate the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR. The difference between them was that in cell SGC7901 vincristine activate the p38 MAPK rapidly whereas in cell SGC7901/VCR slowly. Conclusion Vincristine could affect the activity of p38 MAPK in gastric cancer cell SGC7901 and multidrug resistant cell SGC7901/VCR. |
| Keywords: | p38 MAPK Vincristine Stomach neoplasms Multidrug resistance |
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