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| Involvement of fatty acid-CoA ligase 4 in hepatocellular carcinoma growth： Roles of cyclic AMP and p38 mitogen-activated protein kinase | |
| 引用本文： | Liang YC,Wu CH,Chu JS,Wang CK,Hung LF,Wang YJ,Ho YS,Chang JG,Lin SY. Involvement of fatty acid-CoA ligase 4 in hepatocellular carcinoma growth： Roles of cyclic AMP and p38 mitogen-activated protein kinase[J]. World journal of gastroenterology : WJG, 2005, 11(17): 2557-2563. DOI: 10.3748/wjg.v11.i17.2557 |
| 作者姓名： | Liang YC Wu CH Chu JS Wang CK Hung LF Wang YJ Ho YS Chang JG Lin SY |
| 作者单位： | School of Medical Technology Taipei Medical University Taipei,Taiwan,China,Department of Surgery Taipei Medical University Hospital,Taipei Medical University,Taipei,Taiwan,China,Department of Pathology School of Medicine,Taipei Medical University,Taipei,Taiwan,China,Department of Internal Medicine Taipei Municipal Jen-Ai Hospital,Taipei,Taiwan,China,School of Medical Technology Taipei Medical University,Taipei,Taiwan,China,Department of Environmental and Occupational Health National Cheng Kung University Medical College,Tainan,Taiwan,China,School of Medical Technology Taipei Medical University,Taipei,Taiwan,China,Department of Molecular Medicine and Laboratory Medicine China Medical University Hospital,Taiwan,China,Department of Internal Medicine School of Medicine,Department of Internal Medicine,Taipei Medical University Hospital,Taipei Medical University,Taipei,Taiwan,China |
| 基金项目： | Supported by the National Science Council of Taiwan Chna(NSC 91-2320-B-038-030- and 92-2320-B-038-051-)Taipei Medical University (TMU 91-Y05-A138) |
| 摘 要： | AIM: Fatty acid-CoA ligase 4 (FACL4) is an arachidonate-preferring enzyme which has been shown to be up-regulated in human colon cancer tissues and implicated in the colon tumorigenesis. The purpose of this study was to investigate the role of FACL4 in the human hepatocellular carcinoma (HCC) tumorigenesis and the specific signal pathways involved in this process. METHODS: We investigated the expression and regulation of FACL4 in HCC, adjacent non-tumorous liver tissues, and cell lines. RESULTS: In HCC patients, we demonstrated that FACL4 gene expression was markedly elevated in the cancerous tissues than in the adjacent non-cancerous liver tissues. In addition, several human hepatoma cell lines, including Hep3B and HepG2, expressed high levels of FACL4. Stable overex-pression of FACL4 knockdown plasmids (small interfering RNA, siRNA) to Hep3B cells significantly decreased FACL4 expression and subsequently limited the cell proliferation. Treatment of Hep3B cells with 8-bromo-cAMP and SB203508 (p38 MAPK inhibitor) significantly suppressed the FACL4 expression. CONCLUSION: FACL4 is involved in the HCC tumorigenesis and both cAMP and p38 MAPK pathways are associated with the regulation of FACL4 in HCC. |
| 关 键 词： | 脂肪酸化-CoA连接酶4 肝细胞肿瘤 循环AMP p38 细胞分裂 蛋白激酶 |
| 收稿时间： | 2004-06-24 |
Involvement of fatty acid-CoA ligase 4 in hepatocellular carcinoma growth: roles of cyclic AMP and p38 mitogen-activated protein kinase |
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| Liang Yu-Chih,Wu Chih-Hsiung,Chu Jan-Show,Wang Chung-Kwe,Hung Ling-Fang,Wang Ying-Jan,Ho Yuan-Soon,Chang Jan-Gowth,Lin Shyr-Yi. Involvement of fatty acid-CoA ligase 4 in hepatocellular carcinoma growth: roles of cyclic AMP and p38 mitogen-activated protein kinase[J]. World journal of gastroenterology : WJG, 2005, 11(17): 2557-2563. DOI: 10.3748/wjg.v11.i17.2557 | |
| Authors: | Liang Yu-Chih Wu Chih-Hsiung Chu Jan-Show Wang Chung-Kwe Hung Ling-Fang Wang Ying-Jan Ho Yuan-Soon Chang Jan-Gowth Lin Shyr-Yi |
| Affiliation: | 1. School of Medical Technology, Taipei Medical University, Taipei, Taiwan, China 2. Department of Surgery, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan, China 3. Department of Pathology, School of Medicine, Taipei Medical University, Taipei, Taiwan, China 4. Department of Internal Medicine, Taipei Municipal Jen-Ai Hospital, Taipei, Taiwan, China 5. Department of Environmental and Occupational Health, National Cheng Kung University Medical College, Tainan, Taiwan, China 6. Department of Molecular Medicine and Laboratory Medicine, China Medical University Hospital,Taiwan, China 7. Department of Internal Medicine, School of Medicine;Department of Internal Medicine, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan, China |
| Abstract: | AIM:Fatty acid-CoA ligase 4 (FACL4) is an arachidonate-preferring enzyme which has been shown to be up-regulated in human colon cancer tissues and implicated in the colon tumorigenesis. The purpose of this study was to investigate the role of FACL4 in the human hepatocellular carcinoma (HCC) tumorigenesis and the specific signal pathways involved in this process. METHODS: We investigated the expression and regulation of FACL4 in HCC, adjacent non-tumorous liver tissues, and cell lines. RESULTS: In HCC patients, we demonstrated that FACL4 gene expression was markedly elevated in the cancerous tissues than in the adjacent non-cancerous liver tissues. In addition, several human hepatoma cell lines, including Hep3B and HepG2, expressed high levels of FACL4. Stable overex-pression of FACL4 knockdown plasmids (small interfering RNA, siRNA) to Hep3B cells significantly decreased FACL4 expression and subsequently limited the cell proliferation. Treatment of Hep3B cells with 8-bromo-cAMP and SB203508 (p38 MAPK inhibitor) significantly suppressed the FACL4 expression. CONCLUSION: FACL4 is involved in the HCC tumorigenesis and both cAMP and p38 MAPK pathways are associated with the regulation of FACL4 in HCC. |
| Keywords: | FACL-4 HCC |
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