年龄对大鼠后肢缺血后血管新生的影响及与人抗原R的关系

目的 探讨年龄对大鼠后肢缺血后血管新生的影响及与人抗原R(HuR)的关系.方法 按鼠龄将雄性SD大鼠分为2组:老龄组(18月龄)和青年组(16周龄),每组6只.建立后肢缺血模型,于术后第14天取缺血后的后肢内收肌组织,免疫组织化学法检测CD31在缺血肌肉组织中表达及新生血管数量,用蛋白印迹法检测HuR蛋白表达及用酶联免疫吸附测定法测定血管内皮生长因子(VEGF)含量.结果 老龄组缺血肌肉组织中毛细血管计数明显低于青年组[(64±7)个/mm2比(144±21)个/mm2,P＜0.05],HuR 蛋白表达明显低于青年组[(1.1±0.1)比(1.5±0.4),P＜0.05],VEGF含量也明显低于青年组[(260±24) μg/L比(351±78) μg/L,P＜0.05].结论 年龄对缺血后血管新生有明显影响,年龄越大缺血后血管新生抑制作用越强,这一作用可能与年龄增加后HuR蛋白表达下降,导致VEGF表达下降有关.

Age affects angiogenesis in ischemic hindlimb of rat with decreased human antigen R

Objective To observe the effects of age on angiogenesis in ischemic hindlimb of rat and its re- lationship with human antigen R. Methods Male SD rats were divided into two groups： the elderly group （ 18 months） and young group （ 16 weeks）. Unilateral hindlimb isehemia was deliberately manipulated by excising the entire femoral art and vein in left femoral of animals. After 14 days of surgery, the ischemic and adductor muscle tissues from hindlimb were initiated with immunostain by the endothelial marker CD31. The western blot was used to detect the expression of human antigen R （HuR） protein; vascular endothelial growth factor （VEGF） levels was de- tected by enzyme-linked immunosorbent assay. Results The capillary counts showed that the angiogenesis situation of elderly group was significantly worse than that of the young group （ P 〈 0.05 ）. The HuR protein expression in elderly group was significantly lower than that in the young group （ P 〈 0. 05 ） ; VEGF levels in elderly group de- creased significantly, compared with the young group （P 〈 0.05）. Conclusion The inhibition effect on angiogene- sis may increase when one is getting old, which may be related to the decreasing of HuR protein and a decline in VEGF expression.