辅酶Q10乳剂对阿霉素致小鼠心肌损伤的保护作用

目的:观察辅酶Q10乳剂对阿霉素致小鼠心肌损伤的保护作用,并初步探讨其可能机制?方法:每天分别给予小鼠辅酶Q10胶囊或辅酶Q10乳剂低?中?高剂量,连续5 d,于第3天时腹腔注射阿霉素造成心肌损伤模型,72 h后检测小鼠心电图,测定小鼠心肌组织中乳酸脱氢酶(LDH)?超氧化物歧化酶(SOD)?丙二醛(MDA)和肌酸激酶(CK)的含量,并用免疫组化方法检测p53蛋白含量?结果:辅酶Q10乳剂可改善阿霉素致心肌损伤小鼠心脏组织中LDH?SOD?MDA和CK的含量,但并未发现其存在明显的剂量依赖性?同时辅酶Q10乳剂可降低小鼠心律失常发生率,并抬高QRS波,且其作用优于辅酶Q10胶囊?结论:辅酶Q10乳剂对阿霉素导致的小鼠心肌损伤具有一定的保护作用,且其对降低小鼠心律失常发生率的作用优于辅酶Q10胶囊,其机制可能与其拮抗阿霉素引起的氧自由基损伤有关?

Protective effect of coenzyme Q10 emulsion on myocardial injury in mice induced by adriamycin

Objective:To investigate the protective effect of coenzyme Q10 emulsion on myocardial injury in mice induced by adriamycin and its mechanism. Methods:Coenzyme Q10 capsules and emulsion were administered intragastrically and intravenously to the mice for 5 days,respectively. In the 3rd day adriamycin was given intraperitoneally to establish myocardial injury mice model. After 72h,the level of SOD,CK,MDA and LDH in myocardial cells were tested,as well as the ECG. The level of p53 protein in myocardial cells was assayed by immunohistochemical method cells. Results:Coenzyme Q10 emulsion improved the level of SOD,CK,MDA and LDH in mice myocardial cells,but with no dose-dependency. Also the incidence of arrhythmia was depressed by coenzyme Q10 emulsion. Conclusion:Coenzyme Q10 emulsion has a protective effect on myocardial injury induced by adriamycin,and its mechanism may be related to the antagonism action.