白藜芦醇致敏TRAIL诱导人肝癌细胞凋亡及其相关机制的研究

目的研究白藜芦醇对TRAIL诱导肝癌细胞SMMC-7721凋亡的影响及其机制。方法培养人肝癌细胞株SMMC-7721,分为对照组、TRAIL干预组、Res+TRAIL干预组,进行DNA末端原位标记(TUNEL)染色法作凋亡分析;Western Blot检测白藜芦醇对SMMC-7721细胞Survivn表达的影响。结果对照组SMMC-7721细胞的凋亡指数为(1.78±0.49)%,TRAIL干预组凋亡指数为(9.86±0.53)%,Res+TRAIL干预组中25μmol/L、50μmol/L、100μmol/L浓度Ros凋亡指数分别为(11.30±0.80)%,(20.89±0.69)%和(27.78±1.88)%。除Res+TRAIL干预组(25μmol/L)与TRAIL干预组比较无显著性差异(P0.05),其余各组之间比较差异均有统计学意义(P均0.05)。Western Blot测定结果显示:25μmol/L Ros处理组Survivin/β-actin的值与对照组相比无统计学差异(P0.05);50μmol/L和100μmol/L Res处理组Survivin/β-actin的值较对照组显著降低(P0.01);100μmol/L Res处理组Survivin/β-actin的值较50μmol/L显著降低(P0.01)。结论 TRAIL对SMMC-7721细胞有凋亡诱导作用;Res对TRAIL诱导人肝癌细胞SMMC-7721凋亡有致敏作用,并且这种作用呈剂量依赖关系;其分子机制可能与下调凋亡抑制因子Survivin的表达有关。

Study on the effects of resveratrol on TRAIL-induced apoptosis in human hepatoma cell and its mechanism

Objective It is to observe the influence of resveratrol on TRAIL -induced apoptosis in human hepatoma cell line SMMC-7721 and its mechanism .Methods SMMC-7721 cells were cultured in vitro and divided into three gropus：con-trol group, TRAIL intervention group, resveratrol and TRAIL intervention group .After treatment with TRAIL/resveratrol and TRAIL for 24 hours, TUNEL staining were given to detect the effects of resveratrol on TRAIL -induced apoptosis in human hepatoma cell line SMMC -7721;Western blot technology was performed to detect the expression of apoptosis -related protein survivin in hepatoma apoptotic cells treated by resveratrol .Results The apoptotic rate of SMMC-7721 cells was （1.78 ±0. 49）%in control group, and was （9.86 ±0.53）% in TRAIL intervention group, was （11.30 ±0.80）%, （20.89 ±0. 69）%, （27.78 ±1.88）%respectively after cotreatment with 100 μg/L TRAIL and 25 μmol/L or 50 μmol/L or 100 μmol/L resveratrol for 24 hours in resveratrol and TRAIL intervention group .There was significant difference between every two groups, excepted 25 μmol/L resveratrol intervention group with TRAIL intervention group （P〈0.05）.The results of detec-tion with Western Blot technology indicated that after being treated by 50 μmol/L, 100 μmol/L resveratrol for 24 hours, the value of surviving/β-actin was down-regulated significantly compared with control group （P〈0.01）;and there was signifi-cant difference between 50 μmol/L and 100 μmol/L resveratrol-treated group （P〈0.01）.Conclusion TRAIL can induce apoptosis of human hepatoma SMMC -7721 cells in vitro.Resveratrol can enhance TRAIL -induced apoptosis of SMMC -7721 cells in dose-dependent manner .This enhancement may be related to the inhibition of expression of survivin by resveratrol .