| 微流控芯片非接触电导检测法测定溴吡斯的明片中溴吡斯的明的含量 |
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| 引用本文： | 蔡自由,李永冲,陈缵光. 微流控芯片非接触电导检测法测定溴吡斯的明片中溴吡斯的明的含量[J]. 中国药房, 2011, 0(28): 2668-2670 |
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| 作者姓名： | 蔡自由 李永冲 陈缵光 |
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| 作者单位： | 广东食品药品职业学院;中山大学药学院; |
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| 基金项目： | 国家自然科学基金资助项目(20575080,21075139) |
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| 摘 要： | 目的:建立测定溴吡斯的明片中溴吡斯的明含量的方法。方法:采用微流控芯片非接触电导检测法。在高电场强度条件下利用微流控芯片上特殊的微通道实现组分快速、高效的分离,以非接触电导检测器进行检测;分析缓冲液种类和浓度、添加剂种类和浓度、分离电压和进样时间等因素对分离检测的影响。结果:优化选择1 mmol.L-1 HAc+1 mmol.L-1 NaAc(pH 4.5)缓冲溶液、不加添加剂、分离电压1.80 kV、进样时间10 s时,2 min内可实现溴吡斯的明片中溴吡斯的明含量快速检测。在优化条件下,溴吡斯的明的检测浓度线性范围为10～100μg.mL-1(r=0.999 7),检出限为0.6μg.mL-(1S/N=3),RSD=1.9%,加样回收率为96.6%～100.3%。结论:本方法简便快速,重复性好,可用于溴吡斯的明片的快速检测和药品生产质量控制。
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| 关 键 词： | 微流控芯片 非接触电导检测 溴吡斯的明 含量测定 |
Determination of Pyridostigmine Bromide in Pyridostigmine Bromide Tablets by Microfluidic Chip with Contactless Conductivity Detection |
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| CAI Zi-you,LI Yong-chong CHEN Zuan-guang. Determination of Pyridostigmine Bromide in Pyridostigmine Bromide Tablets by Microfluidic Chip with Contactless Conductivity Detection[J]. China Pharmacy, 2011, 0(28): 2668-2670 |
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| Authors: | CAI Zi-you LI Yong-chong CHEN Zuan-guang |
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| Affiliation: | CAI Zi-you,LI Yong-chong(Guangdong Food and Drug Vocational College,Guangzhou 510520,China) CHEN Zuan-guang(School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China) |
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| Abstract: | OBJECTIVE: To establish the method for the content determination of pyridostigmine bromide(PB) in Pyridostigmine bromide tablets(PBT) by microfluidic chip with contactless conductivity detection.METHODS: Microfluidic chip with contactless conductivity detection was adopted.PB was rapidly separated by microfluidic chip and detected with contactless conductivity detection under high electric field.The electrophoretic parameters,such as the variety and concentration of buffer solution and additive,separation v... |
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| Keywords: | Microfluidic chip Contactless conductivity detection Pyridostigmine bromide Content determination |
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