苘麻子中脂肪酸成分的GC-MS分析

目的: 分析苘麻子中脂肪酸的组成并测定主要脂肪酸成分的含量。方法: 采用索氏提取法提取其脂肪酸,经甲酯化处理后,用GC-MS联用仪对其脂肪酸组成进行分析和鉴定。采用毛细管气相色谱法,甲酯化后以二十一酸甲酯为内标同时测定苘麻子中棕榈酸、硬脂酸、油酸和亚油酸的含量。结果: 共分离出10种脂肪酸,占脂肪油总量的99.43%。棕榈酸、硬脂酸、油酸和亚油酸的线性分别为33.6~336 mg·L^-1(r=0.999 0), 6.58~65.8 mg·L^-1 (r=0.999 9),33.4~334 mg·L^-1 (r=0.999 8),128.3~1 283 mg·L^-1 (r=0.999 5)。各成分的平均回收率分别为100.4%,100.6%,101.6%,101.0%,RSD分别为1.3%,1.5%,1.6%,2.1% (n=9)。结论: 鉴定了苘麻子中的10种脂肪酸并首次建立了其中4种主要脂肪酸成分的含量测定方法,可为苘麻子的质量评价提供依据。

GC-MS Analysis and GC Determination of Fatty Acids in the Seeds of Abutilon theophrasti

Objective: To analyze the components of fatty acids in the seeds of Abutilon theophrasti and develop a method for the simultaneous determination of the main fatty acids. Method: Samples were extracted by Soxhlet extraction. After methyl esterification treatment, fatty acids were identified by GC-MS and determined by GC with a FID detector and methyl heneicosanoate as internal standard. Result: Total 10 kinds of fatty acids were identified, accounted for 99.43% of the total fatty oil. The calibration curve was linear within the range of 33.6-336 mg·L^-1 (r=0.999 0), 6.58-65.8 mg·L^-1 (r=0.999 9), 33.4-334 mg·L^-1 (r=0.999 8), 128.3-1 283 mg·L^-1 (r=0.999 5) for palmitic acid, octadecanoic acid, oleic acid and linoleic acid respectively. The average recoveries (n=9) were 100.4%, 100.6%, 101.6% and 101.0% for palmitic acid, octadecanoic acid, oleic acid and linoleic acid respectively. Conclusion: Ten kinds of fatty acids are identified and a method for the determination of palmitic acid, octadecanoic acid, oleic acid and linoleic acid in Abutilon theophrasti simultaneously is developed. The method is sensitive, accurate and reproducible.