富血小板血浆联合骨髓间充质干细胞不同 配比浓度对兔软骨缺损的影响

目的: 研究富血小板血浆(PRP)与骨髓间充质干细胞(BMSCs)不同的配比浓度对兔软骨缺损的治疗效果。方法: 随机将60只新西兰兔随机分为模型对照组、PRP组(3%)、BMSCs组(1×10⁶/mL)、不同配比浓度的PRP+BMSCs联合组(1:5,2:5,3:5)。建立兔软骨损伤模型,术后第2周靶侧肌肉注射不同配比浓度的PRP与BMSCs,每周1次,连续给药4周。采用Masson's三色染色法,观察关节软骨缺损情况;采用Pineada法进行关节软骨组织学半定量计分,并通过观察患兔术膝切口,了解术后愈合情况。结果: 与模型对照组比较,不同配比浓度的PRP与BMSCs联合组中的关节软骨半定量分值均减少(P<0.05)。不同配比浓度的PRP与BMSCs联合组中,关节软骨半定量分值随PRP浓度的增加而减少,关节软骨半定量分值与PRP值呈现线性关系,其中PRP与BMSCs配比浓度为3:5的效果最为明显,差异有显著性意义(P<0.05)。结论: PRP,BMSCs在一定程度上可促进骨组织恢复;关节软骨缺损恢复程度与PRP浓度表现出一定的量效关系。

Improvement of Rabbit Cartilage Defects by Platelet-rich Plasma Combined with Mesenchymal Stem Cells of Bone Marrow at Different Consistency

Objective: To study effect of platelet rich plasma combined with bone marrow mesenchymal stem cells at different consistency on rabbit cartilage defect. Method: Sixty rabbits were randomly divided into control group, platelet-rich plasma(PRP) group(3%), bone mesenehymal stem cells(BMSCs) group(1×10⁶/mL)and combination group(PRP+BMSCs 1:5, 2:5, 3:5).The models of rabbit cartilage defect were made by operation.Then the models were injected combination at different consistency once a week from 2th week to 5th week after operation. The rabbit cartilage defects were observed by Masson trichrome coloration. Semi-quantitative scoring of articular cartilage were computed by Pineada. Repair of articular cartilage defect in rabbits were observed. Result: Compared with the control group, semi-quantitative scoring of articular cartilage in each combination group decreased(P<0.05). In combination groups, semi-quantitative scoring of articular cartilage was lower when PRP at higher consistency. PRP combined with BMSCs(3:5) was lowest in combination groups(P<0.05). Conclusion: PRP combined with BMSCs can effectively induce bone regeneration and improve bone tissue recovery. The recovery of articular cartilage defects was related with PRP concentration.