| 急性高眼压大鼠视网膜睫状神经营养因子的表达 |
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| 引用本文： | 朱益华,李秀云,唐宝丰,徐国兴,林发森.急性高眼压大鼠视网膜睫状神经营养因子的表达[J].眼视光学杂志,2004,6(2):100-102. |
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| 作者姓名： | 朱益华 李秀云 唐宝丰 徐国兴 林发森 |
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| 作者单位： | 福建医科大学第一附属医院,眼科,福建,福州,350005 |
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| 基金项目： | 福建省科委重大科研项目 (2 0 0 0F0 0 5 ) |
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| 摘 要： | 目的 :探讨大鼠急性高眼压前后睫状神经营养因子 (CNTF)mRNA在视网膜组织中的表达变化及意义。方法 :利用前房高眼压灌注法 ,使大鼠右眼维持在 110mmHg高眼压 30min ,然后恢复视网膜血供 ,并分别于灌注后 1d、3d处死大鼠 ,用半定量RT PCR方法检测大鼠视网膜组织中CNTFmRNA的表达情况。结果 :在正常大鼠视网膜组织中 ,CNTFmRNA有微量表达 ,急性高眼压后 ,其表达明显增高 ,第 1天、第 3天分别比对照组增加 37.0 9%和 2 2 7.4 2 %。结论 :大鼠急性高眼压损伤后 ,可通过内源性CNTF表达增加来应答视网膜神经节细胞 (RGCs)及其他细胞的损伤 ,保护视网膜及视神经 ,为外源性营养因子的应用提供理论依据。
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| 关 键 词： | 急性高眼压/发生 视网膜 睫状神经营养因子/分析 |
| 文章编号： | 1008-1801(2004)02-0100-03 |
| 修稿时间： | 2004年1月8日 |
Expression of CNTF before and after acute high intraocular pressure |
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| ZHU Yi-hua,LI Xiu-yun,TANG Bao-feng,et al..Expression of CNTF before and after acute high intraocular pressure[J].Chinese Journal of Optometry & Ophthalmology,2004,6(2):100-102. |
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| Authors: | ZHU Yi-hua LI Xiu-yun TANG Bao-feng |
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| Institution: | ZHU Yi-hua,LI Xiu-yun,TANG Bao-feng,et al. Department of Ophthalmology,the First Affiliated Hospital,Fujian Medical Univer sity,Fujian China,350005 |
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| Abstract: | Objective: To investigate the gene expression of CNTF in the rat retina in acute high intraocular pressure(AHIOP). Methods: Retinal ischemia was induced by increasing pressure in the anterior cha mber with the perfusion of a normal saline solution in the anterior chamber for 30 min,then restoring blood supply to the retina. The retina was pooled off afte r 1 day and 3 days of reperfusion,and the levels of CNTF mRNA were compared by using a semi-quantitative RT-PCR method. Results: A minimum expression of CNTF mRNA was found in the normal retina. Howev er,there was an increase of 37.09% and 277.42% after 1 day and 3 days of AHIOP. Conclusion:Retinal neurons respond to AHIOP by up-regulation of CNTF mRNA expre ssion,which may offer a theoretical basis for the use of CNTF as a foreign agent . |
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| Keywords: | acute high intraocular pressure/ocurrence retina ciliary neuronotrophic factor /analysis |
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