| 银丹清眩胶囊中黄芪甲苷含量测定方法的建立 |
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| 引用本文： | 周美娟,沈丽琴,杜育华,刘月庆. 银丹清眩胶囊中黄芪甲苷含量测定方法的建立[J]. 江西中医学院学报, 2010, 22(2): 56-57 |
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| 作者姓名： | 周美娟 沈丽琴 杜育华 刘月庆 |
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| 作者单位： | 汇仁集团有限公司,南昌,330000 |
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| 摘 要： | 目的：建立银丹清眩胶囊中黄芪甲苷的含量测定方法.方法：利用高效液相蒸发光散射检测器检测,采用反相ODS柱,流动相：乙腈-水（30∶70）, 流速：1.0 ml/min,柱温：35 ℃,漂移管温度：105 ℃,气体流速：2.8 L/min.结果：黄芪甲苷含量测定的线性范围2.04～20.4 μg,回归方程为Y=1.459 0X＋1.856 8,R2=0.999 7,平均回收率为97.8%,RSD%=1.64%.结论：该方法准确可靠重现性好,可作为样品中黄芪甲苷的含量测定方法.
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| 关 键 词： | 黄芪甲苷 HPLC-ELSD 含量测定 |
Establish the method for determining the content of astragaloside in Yindanqingxian Capsule. |
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| ZHOU Mei-juan,SHEN Li-qin,DU Yu-hua,LIU Yue-qing. Establish the method for determining the content of astragaloside in Yindanqingxian Capsule.[J]. Journal of Jiangxi College of Traditional Chinese Medicine, 2010, 22(2): 56-57 |
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| Authors: | ZHOU Mei-juan SHEN Li-qin DU Yu-hua LIU Yue-qing |
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| Affiliation: | ZHOU Mei-juan,SHEN Li-qin,DU Yu-hua,LIU Yue-qingHuiren Group Co.,Ltd,Nanchang 330000 |
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| Abstract: | Objective：To establish the method for determining the content of astragaloside in pharmaceutics. Methods： HPLC-ELSD was used in the quantitative analysis , by using Reversal ODS chromatography column with aeetonitrile-water（30： 70） as a mobile phase. The flow rate of mobile phase was 1.0 ml/min. The tube temperature of the detector was 105 ~C. The flow rate of pure air was 2.8 L/min. Results ： The regression equation was Y = 1. 4590X ＋ 1. 8568, R2 = 0.9997 （ 2.04 N 20.4 p~g）. The average recovery of astragaloside was 97.8%, and RSD was 1.64%. Conclusion ：The method is reproducible, accurte. It can be used effectively for astragaloside content control of sample. |
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| Keywords: | HPLC-ELSD |
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