Radioimmunoassayable insulin-like growth factor-I in human breast cyst fluid |
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| Affiliation: | 1. Imperial Cancer Research Fund, Department of Clinical Endocrinology, P.O. Box 123, Lincoln''s Inn Fields, London WC2 3PX, U.K.;2. Clinical Oncology Unit, Guy''s Hospital, London SE1 9RT, U.K.;1. Clinical Institute of Medical and Chemical Laboratory Diagnosis, Medical University of Graz, Austria;2. Core Facility Mass Spectrometry, Medical University of Graz, Austria;3. Department of Laboratory Medicine, Institute of Clinical Pathology, Academic Hospital of Udine, Italy;4. Department of Medicine, University of Udine, Italy;5. Department of Clinical Medicine and Surgery, Section of Endocrinology, University Federico II, Naples, Italy;6. UNESCO Chair for Health Education and Sustainable Development, University Federico II, Naples, Italy;7. Department of Experimental Medicine, Policlinico Umberto I Hospital, Sapienza University of Rome, Italy;8. Department of Experimental Medicine, Sapienza University of Rome, Italy;9. Institute of Molecular Biosciences, University of Graz, Austria;1. Department of Laboratory Medicine, St. Michael’s Hospital, Toronto, ON, Canada;2. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada;3. Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA;4. Department of Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada;5. Department of Medicine, Division of Endocrinology and Metabolism, St. Michael’s Hospital, Toronto, ON, Canada;6. The Hospital for Sick Children, Toronto, ON, Canada;7. Li Ka Shing Knowledge Institute, St. Michael’s Hospital, Toronto, ON, Canada |
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| Abstract: | ![]()
Insulin-like growth factor-I (IGF-I) was measured in breast cyst fluid and serum collected at the same time. In addition, epidermal growth factor (EGF) was measured in breast cyst fluid. The radioimmunoassay inhibition curve of cystic IGF-I was parallel to that of authentic IFG-I. Cystic immunoreactive IGF-I had an elution pattern similar to IGF-I from Sep-Pak C18 silica columns and from gel-filtration chromatography using Sephacryl 200. The material from Sephacryl 200 gave a radioimmunoassay curve which was parallel to IGF-I.The median (range) amount of IGF-I in cyst fluid was 9.3 ng/ml (0.8–33.3 ng/ml) and was 5–10% of that found in serum, although there was no significant correlation between the two levels. The median (range) level of cystic EGF was 416 ng/ml (14–1640 ng/ml). There was a weak negative correlation between cystic EGF and cystic IGF-I (P < 0.01).Some women presented with multiple cysts and for these cysts there was a high degree of concordance of levels for both IGF-I or EGF.Unlike serum there was little or no protein present in cyst fluid which bound IGF-I. |
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