HPLC测定冬凌草甲素固态类脂纳米粒在小鼠肝脏中的药物浓度

 目的建立冬凌草甲素固态类脂纳米粒在小鼠肝赃中药物浓度的HPLC 方法小鼠肝脏匀浆液用醋酸乙酯提取,氮气挥干,残渣用甲醇溶解后进样采用Phenomenex-ODS柱(150mm×4.60mm,5μm),以甲醇-水(60:40)为流动相,流速1.0mL·min^-1,检测波长为239nm,外标法定量结果冬凌草甲素的线性范围为0.5～90μg·g^-1(r=0.997 7);最低检测限为20ng; 低、中、高3个浓度的萃取回收率为73.76%～79.08%,方法回收率为85.14%～90.58%,日内精密度≤7.30%,日间精密度≤13.63%,n=5 结论该方法简便、灵敏、准确,适用于冬凌草甲素肝靶向制剂的肝脏药物浓度测定及肝脏靶向性评价。

Determination of oridonin in mice liver following tail intravenous injection of oridonin solid liposome nanoparticles by HPLC

OBJECTIVE To establish HPLC assay for the determination of oridonin concentration in mice liver. METHODS The liver samples were extracted with ethyl acetate and the solvent was evaporated to dryness by N₂ The residue was dissolved by methanol. The separation was performed on a Phenomenex-ODS column(150mm×4.60 mm,5μm)with the mobile phase consisted of methanol water (60:40) at a flow rate of 1.0 mL·min^-1. UV detection wavelength was at 239 nm. RESULTS The calibration curve was linear over the range of 0.5～90μg·g^-1(r=0.9977)and the limit of detection was 20 ng. The extraction recoveries were 73.76% -79.08% (n =5).The recoveries of the assay were 85.14%～90.58% (n=5). The RSDs of intra-day and inter-day didn' t exceed 7.30% and 13.63% (n= 5), respectively. CONCLUSION The present study provided a sensitive, accurate and reliable method for the determination of oridonin in mice liver and the evaluation of hepatic targeting efficiency.