LIPS method for the detection of SARS-CoV-2 antibodies to spike and nucleocapsid proteins |
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| Authors: | Liis Haljasmägi Anu Remm Anna Pauliina Rumm Ekaterina Krassohhina Hanna Sein Anu Tamm Kai Kisand Pärt Peterson |
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| Affiliation: | 1. Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia;2. United Laboratories, Tartu University Hospital, Tartu, Estonia |
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| Abstract: | Profiling antibodies to SARS-CoV-2 can help to assess potential immune response after COVID-19 disease. Luciferase IP system (LIPS) assay is a sensitive method for quantitative detection of antibodies to antigens in their native conformation. We here describe LIPS to detect antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 26 out of 26 COVID-19 patients with N antigen or with three protein fragments when combined into a single reaction. The assay correlated well with ELISA method and was specific to COVID-19 as we saw no reactivity among uninfected healthy controls. Our results show that LIPS is a rapid and measurable method to screen antibody responses against SARS-CoV-2 antigens. |
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| Keywords: | antibodies COVID-19 LIPS luciferase immunoprecipitation SARS-CoV-2 |
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