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| UPLC法测定黄蒲通窍胶囊中二苯乙烯苷的含量 | |
| 引用本文： | 洪燕,韩燕全,陈杨,萧伟. UPLC法测定黄蒲通窍胶囊中二苯乙烯苷的含量[J]. 山西中医学院学报, 2012, 13(5): 28-30 |
| 作者姓名： | 洪燕 韩燕全 陈杨 萧伟 |
| 作者单位： | 1. 安徽中医学院,安徽合肥,230031 2. 安徽中医学院第一附院,国家中医药管理局中药制剂三级实验室,安徽合肥230031 3. 江苏康缘药业股份有限公司,中药制剂过程新技术国家重点实验室,江苏连云港222001 |
| 基金项目： | 安徽省康缘中医药科技创新基金项目 |
| 摘 要： | 目的：建立UPLC法测定黄蒲通窍胶囊中二苯乙烯苷含量的方法。方法：采用超高液相色谱法,色谱柱为AcquityBEH C1（850 mm×2.1 mm,1.7μm）;流动相为乙腈∶水（17∶83）;检测波长：320 nm;柱温：30℃;流速：0.20 mL/min（r=0.999 8）。结果：二苯乙烯苷在0.049 7 mg/mL～0.248 5 mg/mL范围内样品浓度与峰面积呈良好的线性关系。平均加样回收率为98.84%,RSD=2.50%。结论：该法简便、准确、重复性好,可用于黄蒲通窍胶囊中二苯乙烯苷含量的测定。 |
| 关 键 词： | UPLC 黄蒲通窍胶囊 二苯乙烯苷 |
Determination of Stilbene Glucoside Content in Huangpu Tongqiao Capsules by UPLC |
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| Hong Yan , Han Yanquan , Chen Yang , Xiao Wei. Determination of Stilbene Glucoside Content in Huangpu Tongqiao Capsules by UPLC[J]. Journal of Shanxi College of Traditional Chinese Medicine, 2012, 13(5): 28-30 | |
| Authors: | Hong Yan Han Yanquan Chen Yang Xiao Wei |
| Affiliation: | 1.The First Hospital Affiliated to Anhui College of TCM,Hefei Anhui 230031;2.Anhui College of TCM,Hefei Anhui 230031;3.Jiangsu Kannion Pharmaceutical Co.Ltd,Lianyungang Jiangsu 222001) |
| Abstract: | Objective ：To establish UPLC method for determination of stilbene glucoside content in Huangpu Tongqiao capsules.Method：The determination was performed on Acquity BEH C1s（850 mm×2.1 mm,1.7μm） with acetonitrile-water （17：83） as mobile phase.Flow rate was at 0.20 mL/min （r=0.999 8） and detecting wave length was at 320 nm.Column temperature was at 30 ℃.Results：The linear range of stilbene glueoside was at 0.049 7 mg/mL-0.248 5 mg/mL.Average recovery rate was 98.84% （RSD=2.50%）.Conclusion：The method was simple,accurate and reproducible,and could be used for determination of stilbene glueoside content in Huangpu Tongqiao capsules. |
| Keywords: | UPLC Huangpu Tongqiao capsules stilbene glucoside |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |