| 逍遥散对肝郁脾虚证大鼠边缘系统神经元超微结构的影响 |
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| 引用本文： | 梁媛,陈家旭,郭晓玲,岳广欣,杨美娟,葛桂玲.逍遥散对肝郁脾虚证大鼠边缘系统神经元超微结构的影响[J].中华中医药杂志,2009(5). |
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| 作者姓名： | 梁媛 陈家旭 郭晓玲 岳广欣 杨美娟 葛桂玲 |
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| 作者单位： | 北京中医药大学中医诊断系;北京中医药大学组织胚胎学教研室;北京中医药大学电镜室; |
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| 基金项目： | 国家自然科学基金(No.30672578); 高等学校博士学科点专项科研基金(No.2006110801); 教育部留学回国人员科研启动基金资助 |
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| 摘 要： | 目的:探讨慢性束缚应激所致肝郁脾虚证大鼠海马CA1区和杏仁核细胞超微结构的变化,明确逍遥散和6-氰基-7-硝喹啉-2,3-双酮(CNQX)对慢性应激所致肝郁脾虚证的保护作用及其机制。方法:将100只雄性SD大鼠随机分为正常组(A组)、假手术组(B组)、模型组(C组)、逍遥散组(D组)、CNQX组(E组)和逍遥散+CNQX组(F组)。C、D、E、F组大鼠通过连续21d慢性束缚应激建立肝郁脾虚证候模型,D、F组大鼠每天束缚前灌服逍遥散5.32g/kg,E、F组大鼠在第1、4、7、10、13、16、19、21d右侧杏仁核区微量注射α-氨基羟甲基恶唑丙酸(AMPA)受体拮抗剂CNQX0.5μl。第22d处死动物,用透射电镜方法观察海马CA1区和左侧杏仁核神经细胞和突触的形态结构。结果:模型组大鼠海马出现了细胞核变形、线粒体嵴模糊,突触结构不清等,正常组、假手术组和给药各组大鼠CA1区未见明显异常;各组大鼠杏仁核均未见明显异常。结论:逍遥散可改善慢性束缚应激导致的海马CA1区超微结构的损伤,其作用机制可能与CNQX一致。
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| 关 键 词： | 慢性束缚应激 肝郁脾虚 逍遥散 CNQX 海马 杏仁核 电镜 |
Effect of XiaoYaoSan on ultrastructure of neurons in limbic system of rats with liver depression and spleen deficiency syndrome caused by chronic immobilization stress |
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| LIANG Yuan,CHEN Jia-xu,GUO Xiao-ling,YUE Guang-xin,YANG Mei-juan,GE Gui-ling.Effect of XiaoYaoSan on ultrastructure of neurons in limbic system of rats with liver depression and spleen deficiency syndrome caused by chronic immobilization stress[J].China Journal of Traditional Chinese Medicine and Pharmacy,2009(5). |
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| Authors: | LIANG Yuan CHEN Jia-xu GUO Xiao-ling YUE Guang-xin YANG Mei-juan GE Gui-ling |
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| Institution: | LIANG Yuan1,CHEN Jia-xu1,GUO Xiao-ling1,YUE Guang-xin1,YANG Mei-juan2,GE Gui-ling3 (1Department of Chinese Diagnosis,Beijing University of Chinese Medicine,Beijing 100029,China,2Department of Histology , Embryology,3Department of Electron Microscope,China) |
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| Abstract: | Objective:To observe the ultrastructural changes of neurons in hippocampal CA1 area and amygdale of rats with liver depression and spleen deficiency syndrome caused by chronic immobilization stress(CIS), discuss protective effect of XiaoYaoSan and CNQX on liver depression and spleen deficiency syndrome and its mechanism.Methods:100 male SD rats were randomly divided into normal control group(A group),sham-operation group(B group),model group(C group),XiaoYaoSan group(D group),CNQX group(E group)and XaoYaoSa... |
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| Keywords: | Chronic immobilization stress Liver depression and spleen deficiency syndrome XiaoYaoSan CNQX Hippocampus Amygdale Electron microscope |
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