肝癌患者外周血CD4~+CD25~+调节性T细胞的变化及其意义

目的 探讨应用流式细胞术检测肝癌患者外周血中CD4~+CD25~+调节性T细胞的变化及意义.方法 应用三色免疫荧光流式细胞仪测定37例肝癌患者及30例肝硬化患者外周血T细胞亚群CD4~+CD25~+/CD~+比值.采用酶联免疫吸附试验(ELISA)法检测外周血中转化生长因子β1(TGF-B1)的表达水平.结果 肝癌患者外周血CD4~+CD25~+/CD4~+比值较肝硬化患者显著增高,两者比较差异有统计学意义(P<0.05);肝癌患者外周血中CD4~+CD25~+T细胞水平与肝癌原发肿瘤的大小、TGF-βl呈正相关(P<0.05).结论 肝癌患者外周血中CD4~+CD25~+调节性T细胞增多,对肝癌患者具有免疫抑制作用.

The increase of CD4~+CD25~+T cells in peripheral blood may be related to immune suppression and tumor progression in liver cancer patients

Objective To evaluate the changes of CD4~+CD25~+T cells in peripheral blood of liver cancer patients,and observe the relationship between CD4~+CD25~+regulatory T cell and the clinicopathologic characters. Methods 37 patients with liver cancer,30 patients with hepatic cirrhosis were included in this study. The proportion of CD4~+CD25~+T cells population in total T cells was evaluated by flow cytometry analysis. The cytokine production (TGF-βl) was measured by ELISA. Results The population of CD4~+CD25~+T cells in peripheral blood from hepatic carcinoma patients was significantly higher in comparison with that in hepatic cirrhosis patients (P<0. 05 ). The CD4~+CD25~+T cells population in liver cancer patients was positively correlated with the cancer size and TGF-β1 level (P<0.05 ). Conclusion CD4~+CD25~+regulatory T cell is prevalent in liver cancer. It may be responsible for immune suppression in liver cancer patients.