L—精氨酸治疗脊髓损伤的实验研究

目的：本实验旨在观察脊髓损伤早期适当增加局部一氧化氮的生成对脊髓血流动力学及神经功能的影响．方法：采用液压损伤装置致大鼠脊髓闭合性中度损伤，分别静脉注射等渗盐水、Ｄ－精氨酸及一氧化氮的前身物质Ｌ－精氨酸．手术显微镜下观察软脊膜小动脉直径，激光多谱勒技术测量局部血流量，同时对动物神经行为进行综合评分．结果：Ｌ－精氨酸组用药后１５分钟血流逐渐增加，１小时达高峰并超过术前水平；软脊膜小动脉直径平均增加０．１ｍｍ；神经功能恢复较快．与对照组之间的差异具有统计学意义．结论：脊髓损伤早期适时、适量增加局部一氧化氮的产生可改善局部血液供应，对损伤的神经组织产生保护作用

The Effects of L arginine Therapeutics on Experimental Spinal Cord Injury

Aim: To determine wheather early production of nitric oxide has effect on the spinal cord injury. Methods: After close spinal cord modest injury by fluid percussion model, rats received saline, D arginine and L arginine, the precusor of nitric oxide, respectively. Then we observed the diameter of pial artery by operation microscope, measured the regional blood flow by laser Dopple flowmeter and evaluated the neural function of hind limb by Baffour's. Results: We found significant enlargement of pial artery diameter, increase of regional spinal cord blood flow and improvement of behavioral score after receiving L arginine. The other two groups had no effect. Conclusion: These findings indicated suitably enhancing local nitric oxide production could improve tissue survival after spinal cord injury through improving regional blood flow and hemarrheology.