五参素对小鼠脾淋巴细胞内cAMP和cGMP含量的影响及细胞毒性作用

目的 :研究五参素片诱导小鼠脾淋巴细胞内cAMP和cGMP含量改变与细胞毒性关系。方法 :将 5 0只小鼠分为 5组 ,每组 10只 ;实验组设五参素片高、中、低 3种剂量组 ,阳性对照药设环磷酰胺组 ,阴性对照组为生理盐水。结果 :五参素片(由刺五加、斑蝥、冬虫夏草三味中药配制的复方片剂 )具有明显提高小鼠脾脏淋巴细胞内cAMP、cGMP含量和cAMP.McGMP比值的作用 ,与空白对照组相比较差异非常显著 (P <0 0 1) ;环磷酰胺组则具有明显降低作用 ,与空白对照组比较P <0 0 1。NK细胞毒性测定结果也证明五参素片具有明显提高NK细胞毒性作用 ,与空白对照组或环磷酰胺组相比较 ,差异性也非常显著 (P <0 0 1)。结论 :五参素片作为一种新型癌细胞分子调控抑制剂 ,能够明显提高小鼠脾脏淋巴细胞内cAMP、cGMP含量和cAMP.McGMP比值。此结果与NK细胞毒性作用增加可能有密切关系 ,其过程可能与膜受体、蛋白激酶级联放大作用和蛋白磷酸化酶系统有关

Effect and cytotoxicity of Wushensu on cAMP and cGMP content in spleen lymphocytes in mice

Objective: To study the relationship between the changes of Wushensu (WSS) tablets induced cAMP and cGMP content in spleen lymphocytes in mice and cytotoxicity. Methods: Fifty mice were divided into five groups (each group had 10 mice). The experimental groups were divided into high, medium and low doses of WSS tablets; the control groups were cyclophosphamide positive control group and saline negative control group. Results: WSS tablets (a compound tablet which consists of Acanthopanax senticosus Harms, Mylabris and cordyceps sinensis Sacc) had the significant effect of increasing cAMP/cGMP content in spleen lymphocytes in mice and cAMP/cGMP ratio. As compared with the control groups, there was significant difference ( P <0.01); and as compared with the control group, the cyclophosphamide group had significant reducing effect ( P <0.01). The determined results of NK cytotoxicity also showed that the WSS tablets had significant effect in increasing the NK cytotoxicity. As compared with the control group or the cyclophosphamide group, there were also significant differences ( P <0.01).Conclusion: As a new molecular inhibitor for regulating and controlling cancer cells, WSS tablets can significantly increase the cAMP/cGMP content in spleen lymphocytes in mice and cAMP/cGMP ratio. This result may be closely associated with the increasing of NK cytotoxicity, and its process may be associated with membrane receptor, the effect of protein kinases cascade amplification and protein phosphatase system.