妊高征中胎盘血管内皮生长因子的表达及其与胎盘释放血管活性物质的关系

目的 探讨胎盘血管内皮生长因子（ＶＥＧＦ）在妊高征中的表达及其与胎盘释放前列环素（ＰＧＩ２）、血栓素Ａ２（ＴＸＡ２）的关系。方法 分别采用蛋白免疫印渍（Ｗｅｓｔｅｒｎ ｂｌｏｔ）和放免技术测定２５例孕晚期正常妊娠（ＮＬＰ）及２５例妊高征（ＰＩＨ）患者的胎盘ＶＥＧＦ表达及胎盘和血浆中ＰＧＩ２、ＴＸＡ２水平。结果 与ＮＬＰ比较,ＰＩＨ组胎盘ＶＥＧＦ的表达显著降低（Ｐ〈０．０１）;胎盘组织及血浆中ＰＧＩ２水平及ＰＧＩ２／ＴＸＡ２显著下降（Ｐ〈０．０１）,ＴＸＡ２水平显著升高（Ｐ〈０．０１）;ＰＩＨ中胎盘ＶＥＧＦ表达与其产生的ＰＧＩ２、ＰＧＩ２／ＴＸＡ２呈显著正相关（Ｐ〈０．０１）,与ＴＸＡ２呈显著负相关（Ｐ〈０．０１）,并与临床收缩期血压（Ｐ〈０．０１）和舒张期血压（Ｐ〈０．０５）呈显著负相关。结论 胎盘ＶＥＧＦ下降

Expression of vascular endothelial growth factor and its relationship with release of vascular active substances in placentae of patients with pregnancy induced hypertension

ObjectiveTo investigate the expression of placental vascular endothelial growth factor (VEGF) and its relationship with placental release of prostacyclin I 2 (PGI 2) and thromboxane A 2(TXA 2) in patients with pregnancy induced hypertension (PIH).Methods Twenty five patients with PIH and twenty five normal late pregnant (NLP) women in the third trimester were observed in this study The expression of VEGF protein in placenta, the plasma and placental homogenate levels of PGI 2 and TXA 2 were determined by Western blot and radioimmunoassay respectively Results Compared with NLP, the expression of placental VEGF protein was significantly decreased ( P <0 01). Plasma and placental homogenate levels of PGI 2, PGI 2/TXA 2 in patients with PIH were significantly decreased ( P <0 01) while TXA 2 level was increased ( P <0 01). There was a significant positive correlation between placental VEGF and PGI 2 and PGI 2/TXA 2 ( P <0 01). A significant negative correlation was found between placental VEGF and TXA 2 ( P <0 01), systolic ( P <0 01)and diastole blood pressure( P <0 05). Conclusion It is suggested that the reduction of placental VEGF may down regulate local PGI 2/TXA 2, resulting in the imbalance of maternal PGI 2/TXA 2 and dysfunction of endothelial cell