Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani |
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| Authors: | S Kuninaga Tomohide Natsuaki Toru Takeuchi Ryozo Yokosawa |
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| Institution: | (1) Department of General Education, Health Sciences University of Hokkaido, Tohbetsu, Hokkaido 061-02, Japan, JP;(2) Faculty of Agriculture, Utsunomiya University, Mine-machi, Utsunomiya 321, Japan, JP;(3) Hokkaido Central Agricultural Experiment Station, Naganuma, Yubari-gun, Hokkaido 069-13, Japan, JP |
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| Abstract: | Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs)
in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly
variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66 – 100%
for isolates of different subgroups within an AG, and 55 – 96% for isolates of different AGs. In neighbor-joining trees based
on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically
significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of
R. solani may be a valuable tool for identifying AG subgroups of biological significance.
Received: 11 February /16 May 1997 |
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| Keywords: | Rhizoctonia solani Ribosomal DNA ITS sequences Anastomosis group |
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