高浓度EB病毒感染人永生化上皮细胞Hacat系

目的：探讨EB病毒感染上皮细胞的机制。方法：大规模培养绒猴淋巴细胞B958系，从中提取并浓缩EB病毒，用胎儿脐带血淋巴细胞滴定后，以高浓度EB病毒感染人永生化上皮细胞Hacat系，提取Hacat细胞基因组DNA，PCR扩增EB病毒基因组特异性核酸序列Bam HIw片段，Southern blotting及原位杂交验证感染结果。结果：PCR，Southernblotting及原位杂交结果证实高浓度EB病毒能够感染Hacat细胞，但是感染率非常低，结论：EBV对上皮细胞存在CR2或多聚IgA受体介导之外的未知感染途径。

Infection of immortalized human epithelial cell line Hacat with high-concentration Epstein-Barr virus.

OBJECTIVE: To study the mechanism by which Epstein-Barr (EB) virus infects human epithelial cells. METHODS: Large-scale culture of marmoset lymphocytes B958 was carried out to extract and condense EB virus therein. Titrated by lymphocytes from fetal umbilical blood, the EB virus of high concentration was used to infect immortalized human epithelial cell line Hacat, followed by genomic DNA extraction from the Hacat cells and amplification of the special DNA sequence Bam HIw fragments of EBV genomic DNA by PCR. Southern blotting and in situ hybridization were employed to confirm the result of PCR. RESULTS: The results of PCR, Southern blotting and in situ hybridization all indicated that high-concentration EBV could infect Hacat cell line, but the rate of infection was rather low. CONCLUSION: There is an unknown pathway of infection for EBV entry into the epithelial cells other than the mediation by CR2 or pIgR.