HIF-1及NGB,VEGF在脑挫裂伤后促脑血管生成的作用机制

目的探讨HIF-1调控Ngb、VEGF在脑挫裂伤后促血管生成的作用。方法采用蛋白质组学技术比较脑挫裂伤患者和非颅脑创伤患者额叶组织的差异蛋白质,并应用生物信息学技术分析鉴定差异蛋白。然后运用免疫组化和酶联免疫方法分别检测差异蛋白HIF-1、Ngb及VEGF在脑组织以及血清中的表达。结果建立了脑挫裂伤患者和非颅脑创伤患者组脑组织的2-DE图谱,鉴定了7种差异蛋白,其中有HIF-1、Ngb及VEGF。HIF-1、Ngb及VEGF在脑挫裂伤患者脑组织中的表达水平均高于非颅脑创伤患者。HIF-1、Ngb及VEGF在脑挫裂伤患者血清中的表达水平均显著高于非颅脑创伤患者。结论脑挫裂伤后,HIF-1通过调控Ngb和VEGF两个靶基因的释放,在新的脑血管生成过程中发挥了重要作用。HIF-1,Ngb和VEGF在血清中的含量变化,可为临床评估和监测缺氧缺血脑病的严重程度提供参考;对脑挫裂伤的诊断、治疗以及疾病的转归具有重要意义。

Mechanism of action of hypoxia-inducible factor-1, neuroglobin, and vascular endothelial growth factor in promoting cerebral angiogenesis after cerebral contusion and laceration

Objective To investigate the role of neuroglobin (NGB) and vascular endothelial growth factor (VEGF) regulated by hypoxia-inducible factor-1 (HIF-1) in promoting angiogenesis after cerebral contusion and laceration.Methods Proteomic technology was used to compare differentially expressed proteins in the frontal lobe between patients with cerebral contusion and laceration and those with non-traumatic brain injury,and bioinformatics technology was used for the analysis and identification of differentially expressed proteins.Immunohistochemistry and ELISA were used to measure the expression of HIF-1,NGB,and VEGF in cerebral tissue and serum.Results The 2-dimensional gel electrophoresis profile was established for brain tissues from patients with cerebral contu sion and laceration or non-traumatic brain injury,and 7 differentially expressed proteins were identified,including HIF-1,NGB,and VEGF.Immunohistochemistry showed that the patients with cerebral contusion and laceration had higher expression of HIF-1,NGB,and VEGF in brain tissue than those with non-traumatic brain injury.The results of ELISA showed that the patients with cerebral contu sion and laceration had higher expression of HIF-1,NGB,and VEGF in serum than those with non-traumatic brain injury.Conclusions HIF-1 regulates the release of two target genes NGB and VEGF after cerebral contusion and laceration and thus plays an important role in cerebral angiogenesis.Changes in the serum levels of HIF-1,NGB,and VEGF can be used as a reference for clinical evaluation and monitoring of the severity of hypoxic-ischemic encephalopathy.They also have important significance in the diagnosis,treatment,and outcome of cerebral contusion and laceration and await in-depth research.