AFP-L3阳性肝细胞癌患者肿瘤分期和外周血淋巴细胞亚群的研究

目的: 分析小扁豆凝素结合型甲胎蛋白(AFP-L3)阳性的肝细胞癌患者肿瘤分期及外周血淋巴细胞的改变,探讨AFP-L3水平与肿瘤分期及免疫状态的关系。方法:采集60例肝细胞癌患者外周血,检测AFP及AFP-L3含量。根据外周血AFP-L3比例将60例原发性肝细胞癌患者分为2组:AFP-L3(+)组和AFP-L3(-)组。流式细胞术检测外周血中各淋巴细胞亚群(NK、CD4⁺、CD8⁺、CD4/CD8、Treg)的比例。结果:AFP-L3(+)组患者UICC分期Ⅲ+Ⅳ期为29例,AFP-L3(-)组为7例;AFP-L3(+)组患者BCLC分期C+D期为20例,AFP-L3(-)组为3例。AFP-L3(+)组患者发生血管侵犯为17例,远高于AFP-L3(-)组(1例)。AFP-L3(+)组患者外周血中NK细胞比例及CD4/CD8比值下降,CD8⁺T和Treg细胞显著比例上升。结论:外周血AFP-L3阳性的肝癌患者容易发生血管侵犯,且分期较差,可能与淋巴细胞亚群发生变化、免疫系统受到抑制有关。

Tumor stages and peripheral blood lymphocyte subsets in patients with AFP-L3-positive hepatocellular carcinoma

AIM: To analyze the variations of tumor stages and peripheral blood lymphocyte subsets in Lens culinaris agglutinin-reactive alpha-fetoprotein(AFP-L3)-positive hepatocellular carcinoma(HCC) and to explore the relationship between tumor stage,immune state and AFP-L3.METHODS: Peripheral blood of sixty HCC patients was collected.The concentrations of AFP and AFP-L3 were detected.The patients were divided into 2 groups according to the expression of AFP-L3: AFP-L3(+) group and AFP-L3(-) group.The variations of ...