湖北利川黄连不同生长龄期主要生物碱的分布及变化

目的:研究湖北利川黄连不同生长龄期主要生物碱的累积变化过程,为标准化种植提供科学依据.方法:采用紫外分光光度法和HPLC测定黄连有效成分.结果:随着生长龄期的增长,黄连叶片数、根茎干重都在不断的增加；黄连不同部位中总生物碱含量的由大到小的顺序依次是根茎＞须根＞叶片；在黄连2～3龄阶段为黄连生长最旺盛的时期,酪氨酸、总生物碱、小檗碱、黄连碱、巴马汀和小檗碱的含量增长速度最快；在5龄阶段,这4种生物碱含量的大小顺序依次是小檗碱、黄连碱、表小檗碱、巴马汀,且这4种生物碱含量之和占总生物碱含量的比例在不断增加.结论:为黄连的种植管理提供了参考.

Study on Division and Change of Main Alkaloids from Coptis chinensis in Lichuan Hubei at Different Growth Stages

Objective: Through studying the cumulative change process of main alkaloids from Coptis chinensis in Lichuan at different growth stages, provides scientific truth for standardization planting. Method: The effective components of C. chinensis were measured by UV spectrophotometry and HPLC methods. Result: The leafs and dry weight of root of C. chinensis were constantly increasing by the growth of the age growth. The main alkaloids content in different parts of C. chinensis was in the order of root>fibrous root>leaf. C. chinensis was growing fastest during two to three years stages, and the content of the tyrosine, total alkaloids, epiberberine, coptisine, palmatine and berberine was growing fastest too. These four alkaloids content was in the order of epiberberine>coptisine>palmatine>berberine. The proportion was insearsing that the sum content of the four alkaloids accounts for total alkaloids content. Conclusion: The result can be used to provide reference for planting and managing C. chinensis.