|
|||||
|
|
| SARS-CoV基因疫苗的构建及免疫原性初步研究 | |
| 引用本文： | 吴雪琼,栾翔凌,熊志红,张俊仙,王宾. SARS-CoV基因疫苗的构建及免疫原性初步研究[J]. 中国现代医学杂志, 2008, 18(5): 531-535 |
| 作者姓名： | 吴雪琼 栾翔凌 熊志红 张俊仙 王宾 |
| 作者单位： | 1. 解放军总医院第二附属医院,全军结核病研究所,北京,100091 2. 中国农业大学,北京,100816 |
| 基金项目： | 解放军总医院第二附属医院科研基金 |
| 摘 要： | 目的构建SARS病毒E、M、N基因疫苗,研究其免疫原性。方法通过PCR扩增获得SARS-CoVE、M、N基因片断,将其分别克隆入真核表达载体pVAX1,将所得真核表达质粒pVAX1-E、pVAX1-M和pVAX1-N分别肌肉注射小鼠和兔子,用ELISA法检测血清抗体,观察实验期间动物的体重变化,处死后对重要脏器进行病理检查。结果pVAX1-E、pVAX1-M和pVAX1-N基因测序结果与基因库中公布的一致。3种基因疫苗肌肉注射免疫小鼠和兔子后均能诱导产生特异性抗SARS-CoV抗体;动物一般状况和体重变化与对照组差异无显著性;重要脏器无明显病理改变。结论pVAX1-E、pVAX1-M和pVAX1-N疫苗能够诱导机体产生特异的体液免疫应答,具有较好的免疫原性和安全性,为SARS疫苗的研制奠定了良好基础。 |
| 关 键 词： | 真核表达 DNA疫苗 免疫原性 SAPS 冠状病毒 基因疫苗 免疫原性 研究 immunogenicity evaluation vaccines SARS 安全性 体液免疫应答 机体 病理改变 差异 对照组 一般状况 血清抗体 注射免疫 肌肉 基因库 基因测序 结果 |
| 文章编号： | 1005-8982(2008)05-0531-04 |
| 修稿时间： | 2007-10-11 |
Development of SARS-CoV vaccines and evaluation of their immunogenicity |
|
| WU Xue-qiong,LUAN Xiang-ling,XIONG Zhi-hong,ZHANG Jun-xian,WANG Bin. Development of SARS-CoV vaccines and evaluation of their immunogenicity[J]. China Journal of Modern Medicine, 2008, 18(5): 531-535 | |
| Authors: | WU Xue-qiong LUAN Xiang-ling XIONG Zhi-hong ZHANG Jun-xian WANG Bin |
| Affiliation: | WU Xue-qiong1,LUAN Xiang-ling1,XIONG Zhi-hong1,ZHANG Jun-xian1,WANG Bin2 (1.Institute of Tuberculosis Research,The Second Affiliated Hospital of Chinese PLA General Hospital,Beijing 100091,P.R.China,2.China Agricultural University,Beijing 100816,P.R.China) |
| Abstract: | [Objective] To develop vaccines of envelop gene(E), membrane gene(M) and nucleocapsid(N) gene from SARS-CoV, and to evaluate their immunogenicity. [Method] E, M and N genes were obtained by PCR, and then cloned into eukaryotic expression vector pVAX1. Mice and rabbits were immunized by intramuscular injection with the recombinant plasmids. The antigen-specific antibodies were determined by ELISA. The weight change of the animals were observed. Their organs were taken, and pathological changes were observed.... |
| Keywords: | eukaryotic expression DNA vaccine immunogenicity SARS coronavirus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |