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| 协同凝集试验快速检出水中痢疾杆菌的初步研究和应用 | |
| 引用本文： | 张符光 陈征余. 协同凝集试验快速检出水中痢疾杆菌的初步研究和应用[J]. 解放军预防医学杂志, 1992, 10(4): 260-265 |
| 作者姓名： | 张符光 陈征余 |
| 作者单位： | 军事医学科学院卫生学环境医学研究所 天津(张符光，陈征途，晁福寰，欧阳川，芮期义，王新为)，军事医学科学院卫生学环境医学研究所 天津(宋农) |
| 摘 要： | 为了快速检出水中的痢疾杆菌,我们以宋内氏痢疾杆菌为模型,采用金黄色葡萄球菌协同凝集试验作为检出方法,并与直接凝集试验,玻片固相抗体吸附免疫荧光技术和常规分离培养法进行了比较研究,对30份天然河塘水进行了应用检验。结果表明,协同凝集试验检出河塘水中的痢疾杆菌较常规培养法和直接凝集法敏感性高,检出阳性率高,方法简便快速,24h即可报告结果。协同凝集试验与玻片固相抗体吸附免疫荧光技术相配合,可提高结果的准确性。 |
| 关 键 词： | 协同凝集 痢疾杆菌 快速检验 |
STAPHYLOCOCCUS AUREUS CO-AGGLUTINATION TEST FOR RAPID DETECTION OF SHIGELLA IN WATER |
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| Zhang Fuguang,Chen Zhengtu,Chao Fuhuan,Oyang Chuan,Rui Qiyi,Wang Xinwei,Song Nong Institute of Hygiene and Environmental Medicine,Academy of Military Medical Sciences,Tianjin. STAPHYLOCOCCUS AUREUS CO-AGGLUTINATION TEST FOR RAPID DETECTION OF SHIGELLA IN WATER[J]. Journal of Preventive Medicine of Chinese People's Liberation Army, 1992, 10(4): 260-265 | |
| Authors: | Zhang Fuguang Chen Zhengtu Chao Fuhuan Oyang Chuan Rui Qiyi Wang Xinwei Song Nong Institute of Hygiene Environmental Medicine Academy of Military Medical Sciences Tianjin |
| Abstract: | Staphylococcus Aureus Co-Agglutination Test (SACAT) was used forrapid detection of Shigella in water. The results were compared with otherthree kinds of methods (i. e. Direct Agglutination (DA), Solid-Phase Antibo-dy Adsorption Immunofluorescent Technique (SPAIF) and routine culturemethod). The results demonstrated that: 1. SACAT show higher positive re-sults than routine culture method alld DA, 2. The percentage of agreementwas contented between SACAT and routine culture method, 3. SACAT issimpler, the results can be obtained in 24 hours. 4. SACAT is suitable todetect Shigella in water. |
| Keywords: | co-agglutioation shigella rapid detection |
| 本文献已被 CNKI 维普 等数据库收录！ | |