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| 丙酮酸乙酯调节脓毒症小鼠肝细胞HSP70表达的研究 | |
| 引用本文： | 陈敏英,陈娟,欧阳彬,管向东. 丙酮酸乙酯调节脓毒症小鼠肝细胞HSP70表达的研究[J]. 中华普通外科学文献(电子版), 2008, 2(5): 20-21 |
| 作者姓名： | 陈敏英 陈娟 欧阳彬 管向东 |
| 作者单位： | 中山大学附属第一医院外科重症监护中心, 广州,510080 |
| 摘 要： | 目的研究丙酮酸乙酯（EP）对脓毒症小鼠肝细胞热休克蛋白70（HSPT0）的调节作用。方法制作小鼠盲肠结扎穿孔模型（CLP），应用丙酮酸乙酯林格氏液（REPS）与乳酸钠林格氏液（RLS）对小鼠进行液体复苏，60只小鼠分3组，各20只：假手术组、CLP模型＋REPS复苏组、CLP模型＋RLS复苏组，检测肝组织丙二醛（MDA）及肝细胞HSP70的表达。结果脓毒症小鼠较假手术组MDA浓度增高，P〈0．01。EP显著提高脓毒症小鼠肝组织的抗氧化能力，REPS组肝组织MDA浓度低于RLS组【（48．18±598）μmol／g．prot vs（78．34±11．16）μmol／gprot，P〈0．01]；REPS组小鼠肝细胞HSPT0表达较RLS组增高[（28．76±5．69）vs（20．04±4．93），P〈0．051。HSP70表达与MDA值呈负相关（r=-0．733，P〈0．01）。结论EP具有的抗氧化作用能提高脓毒症小鼠肝细胞的HSPT0表达。 |
| 关 键 词： | 脓毒症 丙酮酸乙酯 热休克蛋白70 |
Modulation of hepatocyte heat shock protein 70 expression by ethyl pyruvate in septic mice |
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| CHEN Min-ying,CHEN Juan,OU Yang-bin,GUAN Xiang-dong. Modulation of hepatocyte heat shock protein 70 expression by ethyl pyruvate in septic mice[J]. Chinese Journal of General Surgery(Electronic Version), 2008, 2(5): 20-21 | |
| Authors: | CHEN Min-ying CHEN Juan OU Yang-bin GUAN Xiang-dong |
| Affiliation: | CHEN Min-ying,CHEN Juan,OU Yang-bin,GUAN Xiang-dong,Department of Surgical Intensive Care Unit,The First Affiliated Hospital of SunYat-sen University,Guangzhou 510080,China |
| Abstract: | Objective To study the modulation of heat shock protein 70(HSP70) expression by ethyl pyruvate(EP) in the hepatocyte of septic mice. Methods Cecal ligation-perforation (CLP) models were made in mice as septic model. Total of 60 mice were divided into three groups: sham group, CLP+REPS group and CLP+RLS group. Ringer's ethyl pyruvate solution (REPS) and Ringer's lactic solution (RLS) were used in two groups to resuscitate the septic mice respectively. Malonaldehyde(MDA) in hepatic tissue and hepatocyte HSP70... |
| Keywords: | Sepsis Ethyl pyruvate Heat shock protein 70 |
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