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| 高效液相色谱法测定复方丹皮汤中丹皮酚含量 | |
| 引用本文： | 韦劲,彭代银,方朝晖,吴德玲.高效液相色谱法测定复方丹皮汤中丹皮酚含量[J].安徽中医学院学报,2009,28(5):75-77. |
| 作者姓名： | 韦劲 彭代银 方朝晖 吴德玲 |
| 作者单位： | 1. 铜陵有色职工总医院药剂科,安徽,铜陵,244000 2. 安徽中医学院 3. 安徽中医学院第一附属医院 |
| 摘 要： | 目的：运用高效液相色谱法检测复方丹皮汤中丹皮酚的含量。方法：选用岛津VP—ODS柱（4．6mm×150mm，5μm）；流动相：甲醇-水（50：50）；流速：1．00ml／min；检测波长：274nm；柱温：30℃.结果：丹皮酚浓度为5．36～26．8μg／ml时与峰面积呈良好的线性关系（r=0．9997），平均回收率为99．12％（n=5），RSD1．25％。结论：运用高效液相色谱法检测复方丹皮汤中丹皮酚的含量简便、准确、重复性好，可作为复方丹皮汤的一种质量控制方法。 |
| 关 键 词： | 复方丹皮汤 丹皮酚 含量测定 高效液相色谱法 |
Content Determination of Paeonol in Compound Cortex Moutan Decoction by High Performance Liquid Chromatography |
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| WEI Jin,PENG Dai-yin,FANG Zhao-hui,WU De-ling.Content Determination of Paeonol in Compound Cortex Moutan Decoction by High Performance Liquid Chromatography[J].Journal of Anhui Traditional Chinese Medical College,2009,28(5):75-77. | |
| Authors: | WEI Jin PENG Dai-yin FANG Zhao-hui WU De-ling |
| Institution: | WEI Jin, PENG Dai yin, FANG Zhao-kui, WU De-ling (Department of Pharmacy, General Workers＇ Hospital of Tongling Nonferrous Melals Corporation, Anhui Tongling 244000, China) |
| Abstract: | Objective：To determine the content of paeonol in Compound Cortex Moutan Decoction by high performance liquid chromatography （HPLC） method. Methods： The samples were analyzed by a VP-ODS column, with mobile phase methanol-water （50： 50） at flow rate of 1.00 ml/min, detection at wavelength of 274 nm and the temperature at 30℃. Results： The calibration curves of paeonol were in good linearity over the range of 5.36～26.8μg/ml （r=0.999 7）. The average recovery rate was 99.12% （n=5） and RSDwas 1.25%. Conclusion： The HPLC method is simple, accurate and reproducible, and it can be used for the quality control standard of paeonol in Compound Cortex Moutan Decoction. |
| Keywords: | Compound Cortex Moutan Decoction Paeonol Content determination High performance liquid chromatography |
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