过氧乙酸对枯草杆菌黑色变种芽胞杀灭机理的研究

<正> 过氧乙酸是目前国内所使用的消毒剂中杀菌作用最强的一种。尽管在卫生防疫工作中得到了较广泛的运用,但对其杀菌机理还不完全清楚。Merk等的实验证实,过氧乙酸能灭活枯草杆菌芽胞的过氧化氢酶和过氧化物酶

STUDY OF BACTERICIDAL MECHANISM OF PERACETIC ACID AGAINST SPORES OF BACILLUS SUBTILIS VAR. NIGER

Spores of Ba cillus subtilis var. niger were exposed to 0.2%, 1% and 2% peracetic acid at 25℃. The structural changes induced by peracetic acid were examined with electronmicroscope. The ultrastructure of spores showed not only the progressive disintegr ation of the coat and cortex, but also disintegration and lysis of the core.Spores of B. subtilis var. niger were labeled by ~3H-TdR, ~3H-Urd or ~3H-Leu in the synthetic medium, then exposed to 0.2%, 1% or 2% peracetic acid at appropriate intervals. The radioactivity of their filtrates and the amount of dipicolinic acid (DPA) of the spores were measured. The results showed that the spore constituents, such as DNA, RNA, DPA and amino acids leaked out and the leakages were inversely proportional to the number of survived spores.The above results suggest that peracetic acid damages the permeability barrier of spore and destroys the cytoplasm of spore core. This may be the mechanism of the bactericidal effect of peracetic acid.