| 沉默SUV39H1基因对急性髓系白血病KG-1细胞凋亡的影响 |
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| 引用本文： | 马旭东,赵婷,黄轶群.沉默SUV39H1基因对急性髓系白血病KG-1细胞凋亡的影响[J].中国实验血液学杂志,2013,21(1):82-86. |
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| 作者姓名： | 马旭东 赵婷 黄轶群 |
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| 作者单位： | 福建医科大学附属漳州市医院血液科,福建漳州,363000 |
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| 基金项目： | 卫生部科学研究基金 福建卫生教育联合攻关计划项目资助，福建省自然科学基金，漳州市科学研究发展计划基金资助项目 |
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| 摘 要： | 摘要本研究旨在探讨沉默SUV39H1基因对急性髓系白血病细胞株KG-1增殖和凋亡的影响。将SUV39HIsiRNA经Lipofectamine…2000转染至KG-1细胞，应用MTS法检测细胞增殖率，观察sUV39111siRNA对KG-1细胞增殖的影响；流式细胞术分析细胞凋亡；Westernblot检测SUV39H1siRNA作用后P15和凋亡相关蛋白BCL-2、procaspase-9、procaspase-3、C-MYC的表达。结果表明，沉默SUV39H1基因可抑制细胞增殖，SUV39H1siRNA浓度为30、60、120、240nmol／L作用48h后，KG-1细胞的增殖率分别为（76．43±1．98）％、（51．31±1．84）％、（37．31±1．61）％、（18．94±3．22）％，差异具有统计学显著意义（P〈0．05）；SUV39HIsiRNA可上调P15基因的表达；SUV39H1siRNA可诱导细胞凋亡，30、60、120nmoL／LSUV39H1siRNA作用48h后，KG-1细胞凋亡率分别为（40．2±5．1）％、（56．8±4．8）％、（71．6±5．6）％，差异有统计学显著意义（P〈0．05）；抗凋亡相关蛋白BCL-2、procaspase-9、procaspase-3、C-MYC的表达减少。结论：SUV39H1siRNA能抑制KG-1细胞的增殖并诱导其凋亡，有望成为白血病治疗的-个新的靶点。
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| 关 键 词： | 急性白血病 SUV39H1 RNA干扰 KG-1细胞 |
Effect of SUV39H1 siRNA Silence on Apoptosis and Proliferation of Acute Myelogenous Leukemia KG-1 Cell Line |
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| MA Xu-Dong
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ZHAO Ting
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HUANG Yi-Qun.Effect of SUV39H1 siRNA Silence on Apoptosis and Proliferation of Acute Myelogenous Leukemia KG-1 Cell Line[J].Journal of Experimental Hematology,2013,21(1):82-86. |
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| Authors: | MA Xu-Dong ZHAO Ting HUANG Yi-Qun |
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| Institution: | Department of Hematology,Zhangzhou Affiliated Hospital of Fujian Medical University,Zhangzhou 363000,Fujian Province,China |
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| Abstract: | This study was aimed to investigate the effects of SUVJ9H1 siRNA on proliferation and apoptosis of acute myelogenous leukemia KG-1 cell line. The small interfering RNA（siRNA） targeting SUV39H] gene was designed and transfected into KG-1 cells by LipofectamineTM2000. Cell growth affected by SUVJ9H1 siRNA was determined by MTS method. Cell apoptosis was measured by flow cytometry. The expressions of P15 and anti-apoptosis protein such as BCL-2, procaspase-9, procaspase-3 and C-MYC were detected by Western blot. The results indicated that siRNA targeting SUV39H! inhibited proliferation of KG-1 cells. Proliferated rates were （ 76.43±1. 98 ） %, （ 51. 31±1. 84） %, （37.31 ±1.61 ） %, （ 18.94 ±3.22） % respectively after transfection with SUV39H1 siRNA at 30, 60, 120, 240 nmol/L for 48 h, while P15 expression was upregulated. Apoptotic cells significantly increased, apoptotic rates were （40.2 ± 5. 1 ） % , （ 56.8 ± 4.8） %, （ 71.6± 5.6 ） % respectively after transfecfion with siRNA targeting SUV39H1 at 30, 60, 120 nmol/L（P 〈 0.05）. The protein expression of BCL-2, procaspase-9, procaspase-3, C-MYC was downregulated after transfection. It is concluded that the siRNA targeting SUV39H1 inhibites cell growth and induces cell apoptosis of KG-1 cell line, which may be a new therapeutic target in human leukemia. |
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| Keywords: | acute leukemia SUV39H1 RNA interference KG-1 cell |
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