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重组人内皮抑素在酵母中的表达及对人肺腺癌Astc-a-1细胞生长的抑制
摘    要:

关 键 词:肺腺癌 重组人内皮抑素 酵母 毕赤酵母系统 重组人内皮抑素

Expression of recombinant human endostatin in Pichia pastoris and its inhibitory effects on the growth of human lungadenocarcinoma Astc-a-1 cells]
Lin Zhang,An-qiao Song,Jin-xu Wen. Expression of recombinant human endostatin in Pichia pastoris and its inhibitory effects on the growth of human lungadenocarcinoma Astc-a-1 cells][J]. Journal of First Military Medical University, 2003, 23(9): 895-898
Authors:Lin Zhang  An-qiao Song  Jin-xu Wen
Affiliation:Department of Respiratory Disease, 309 Hospital of PLA, Beijing 100091, China.
Abstract:OBJECTIVE: To achieve expression of recombinant human endostatin (rhES) in a highly efficient foreign gene expression system, the yeast strain Pichia pastoris, and to evaluate the inhibitory effect of rhES on the growth of nude mouse pulmonary adenocarcinoma cells. METHODS AND RESULTS: The rhES gene was efficiently expressed in the yeast strain, and heparin affinity chromatography yielded highly purified endostatin identified by Western blotting, which proved to significantly inhibit the growth of the ECV-304 cells in vitro and also the growth of the lung adenocarcinoma Astc-a-1 in nude mice. CONCLUSIONS: The rhES gene can be expressed in Pichia pastoris, and has the ability to restrain the growth of ECV-304 cells and lung adenocarcinoma Astc-a-1 in nude mice, showing important potentials for future clinical applications.
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