Studies on the A, B, O(H) Blood Groups on Human Cells in Culture |
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| Authors: | CHESSIN LAWRENCE N; BRAMSON SHARON; KUHNS WILLIAM J; HIRSCHHORN KURT |
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| Institution: | 1 Departments of Medicine and Pathology, New York University School ofMedicine, and the III and IV (N. Y. U.) Medical Divisions, Bellevue Hospital, New York,N. Y.
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| Abstract: | HeLa cells were used in the mixed agglutination reaction to determineoptimal conditions for demonstrating blood group H activity by this method.The following parameters were studied in the mixed agglutination reaction:(1) derivation of cell line, (2) cell viability, (3) effects of antibody titer, (4)source and type of antibody.Studies with primary human amnion cells indicated that over a 30-day period of cultivation in vitro there were losses in specific ABO blood groupactivity.Addition of blood group precursors to establish human amnion celllines FL-J and F-D indicated that blood group B antigen could be synthesizedand maintained in vitro. Submitted on September 8, 1964 Accepted on October 26, 1964 |
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