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| 小鼠阴道黏膜干细胞生长相关因子的初步研究 | |
| 引用本文： | 梁晓萍,王沂峰,刘风华. 小鼠阴道黏膜干细胞生长相关因子的初步研究[J]. 中国临床保健杂志, 2006, 9(6): 595-596 |
| 作者姓名： | 梁晓萍 王沂峰 刘风华 |
| 作者单位： | 安徽医科大学附属省立医院、安徽省立医院妇产科,合肥,230001;广州市第二人民医院 |
| 基金项目： | 广东省科技攻关计划课题(粤卫〔2004〕115号) |
| 摘 要： | 目的对小鼠阴道黏膜干细胞培养上清中的细胞因子进行初步鉴定。方法收集稳定传代后的小鼠阴道黏膜干细胞培养上清,进行SDS-PAGE电泳和W estern-b lot检测,鉴定培养上清中阴道黏膜干细胞分泌的细胞因子。结果小鼠阴道黏膜干细胞培养上清中含有一种分子量约为84KD的细胞因子,经W estern-b lot方法检测该细胞因子为干细胞生长因子(HGF)。结论小鼠阴道黏膜干细胞可以分泌有活性的HGF。 |
| 关 键 词： | 生长物质 干细胞 黏膜 动物实验 |
| 文章编号： | 1672-6790(2006)06-0595-02 |
| 收稿时间： | 2006-06-28 |
| 修稿时间： | 2006-06-28 |
Study on mouse vaginal mucous membrane stemcell-related growth factor |
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| LIANG Xiaoping,WANG Yifeng,LIU Fenghua. Study on mouse vaginal mucous membrane stemcell-related growth factor[J]. Chinese Journal of Clinical Healthcare, 2006, 9(6): 595-596 | |
| Authors: | LIANG Xiaoping WANG Yifeng LIU Fenghua |
| Abstract: | Objective To identify the cytokines in the supernatant of mouse vaginal mucous membrane stem cells.Method To collected the supernatant of mouse vaginal mucous membrane stem cells after VMSCs were subcultured steadly,to detecte cytokines secreted by mouse vaginal mucous menbrance stem cells by SDS-PAGE cataphoresis and Western-bloting methods.Result One kind of cell grath factor with 84kd molecular weight was identified by Western-bloting which was hepatocyte growth factor(HGF).Conclusion VMSCs can secrete active HGF. |
| Keywords: | Growth substances Stem cells Mucous membrane Animal experimentation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |